Heteroplasmy of mitochondrial DNA in the ophiuroid Astrobrachion constrictum
Heteroplasmy of mitochondrial DNA in the ophiuroid Astrobrachion constrictum
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DOI：
10.1093/jhered/91.2.146
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发表时间：
2000-03-01
影响因子：
3.1
通讯作者：
Wallis, GP
中科院分区：
文献类型：
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作者：
Steel, DJ;Trewick, SA;Wallis, GP
我们证明存在线粒体异质性的细胞色素氧化酶I（COI）基因的脆性星星（收缩星。分析的117个个体中有一个包含两个不同的单链构象多态性（SSCP）单倍型，其差异在于两个取代;另一个显示了异质性的序列证据。我们使用聚合酶链反应（PCR）克隆、SSCP和COI 5′端480 bp区域的测序来分离和表征这些单倍型。这是第一个正确证实的棘皮动物物种异质性的病例，可能是由父系泄漏引起的。
We demonstrate the presence of mitochondrial heteroplasmy for the cytochrome oxidase I (COI) gene of the brittle star (Astrobrachion constrictum. One of the 117 individuals analyzed contained two distinct single-strand conformation polymorphism (SSCP) haplotypes differing by two substitutions; another showed sequence evidence for heteroplasmy. We used polymerase chain reaction (PCR) cloning, SSCP, and sequencing of a 480 bp region of the 5′ end of COI to isolate and characterize these haplotypes. This is the first properly substantiated case of heteroplasmy in an echinoderm species and may have arisen from paternal leakage.