Acupuncture stimulation attenuates TNF-α production via vagal modulation in the concanavalin A model of hepatitis
Acupuncture stimulation attenuates TNF-α production via vagal modulation in the concanavalin A model of hepatitis
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DOI：
10.1177/0964528420907338
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发表时间：
2020-12-01
影响因子：
2.5
通讯作者：
Namgung, Uk
中科院分区：
文献类型：
--
作者：
Lim, Hee-Don;Kim, Ki-Joong;Namgung, Uk
背景：越来越多的证据表明，神经元活动参与调节针灸治疗的疗效。目的：采用刀豆蛋白A（ConA）肝炎模型，研究针刺足三里穴对肝组织和血液中炎性细胞因子肿瘤坏死因子（TNF）-α的调节作用。方法：针刺足三里穴，针刺足三里穴，切断迷走神经。收集肝组织和血液用于TNF-α分析。结果：ConA注射后6-24 h，CD 11b阳性肝细胞和血浆中TNF-α mRNA和蛋白表达明显增加，CD 11b、CD 68和Erk 1/2蛋白表达明显增加。MA或EA的应用在减弱TNF-α的产生方面非常有效。在ConA注射动物中，VNX极大地抑制了针刺的抗炎作用，这表明针刺介导的抗炎反应需要迷走神经活性。坐骨神经（SNS）的电刺激导致类似于针刺刺激的抗炎作用。结论：针刺刺激对肝脏炎症反应的调节作用可能是通过激活迷走神经通路来调节的。
Background:A growing body of evidence shows that neuronal activity is involved in modulating the efficacy of acupuncture therapy. However, it has been seldom investigated whether neuronal activity following acupuncture stimulation is effective at regulating hepatic inflammation.Objective:Using the concanavalin A (ConA) model of hepatitis, we investigated the regulation of inflammatory cytokine tumor necrosis factor (TNF)-alpha in the liver tissue and the blood after acupuncture stimulation at ST36.Methods:Mice were subjected to ConA injection, acupuncture stimulation at ST36 by manual acupuncture (MA) or electroacupuncture (EA) procedures, and vagotomy (VNX). Liver tissue and blood were collected for TNF-alpha analysis. TNF-alpha mRNA was analyzed by real-time polymerase chain reaction (PCR), and TNF-alpha, CD11b, CD68, and Erk1/2 proteins were analyzed by Western blotting, immunofluorescence staining, and enzyme-linked immunosorbent assay.Results:TNF-alpha mRNA and protein were induced in CD11b-positive hepatic cells and the plasma at 6-24 h after ConA injection. The application of MA or EA was very effective at attenuating the production of TNF-alpha. Anti-inflammatory effects of acupuncture were greatly suppressed by VNX in ConA-injected animals, suggesting the requirement of vagus nerve activity in acupuncture-mediated anti-inflammatory responses. Electrical stimulation of the sciatic nerve (SNS) resulted in an anti-inflammatory effect similar to acupuncture stimulation. In parallel with TNF-alpha, production of phospho-Erk1/2, which was induced in the liver tissue, was downregulated by MA and EA in liver cells.Conclusion:The regulatory effects of acupuncture stimulation on inflammatory responses in the liver may be modulated through the activation of the vagus nerve pathway.