Effect of Activated Carbon on Microbial Bioavailability of Phenanthrene in Soils
Effect of Activated Carbon on Microbial Bioavailability of Phenanthrene in Soils
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DOI：
10.1897/09-081.1
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发表时间：
2009-11
影响因子：
4.1
通讯作者：
Yu Yang;W. Hunter;S. Tao;D. Crowley;J. Gan
中科院分区：
文献类型：
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作者：
Yu Yang;W. Hunter;S. Tao;D. Crowley;J. Gan
生物有效性是控制土壤中疏水性有机污染物生物降解速率的主导因素。在土壤中吸附疏水性有机污染物的固相中，黑碳（BC）对相态分布的影响尤为显著。然而，关于BC对土壤中多环芳烃微生物有效性的影响的知识仍然有限。在本研究中，在三种土壤中测量了煤衍生活性炭对范氏分枝杆菌PYR-1降解菲（PHE）过程中菲生物利用度的影响。自由溶解的PHE的浓度同时测定在土壤溶液中使用一次性聚二甲基硅氧烷纤维。结果表明，添加活性炭后，所有供试土壤中菲的矿化均受到显著抑制。216小时后，只有5.20，5.83，和6.85%的苯丙氨酸降解0.5%的BC-修正的土壤中，最初含有的有机碳分别为0.23，2.1，和7.1%。菲的降解率与自由溶解浓度之间存在显著相关性，表明BC通过降低化学活性影响菲的生物有效性。活性炭的作用主要是通过增加土壤表面积和孔隙体积来实现的。本研究的结果清楚地强调了BC对影响土壤中多环芳烃微生物有效性的重要性。
Bioavailability is a governing factor that controls the rate of biological degradation of hydrophobic organic contaminants in soil. Among the solid phases that can adsorb hydrophobic organic contaminants in soil, black carbon (BC) exerts a particularly significant effect on phase distribution. However, knowledge on the effect of BC on the microbial availability of polycyclic aromatic hydrocarbons in soil is still limited. In the present study, the effect of a coal‐derived activated carbon on the bioavailability of phenanthrene (PHE) during its degradation by Mycobacterium vanbaalenii PYR‐1 was measured in three soils. The freely dissolved concentration of PHE was concurrently determined in soil solutions using disposable polydimethylsiloxane fibers. The results showed that PHE mineralization was significantly inhibited after addition of activated carbon in all test soils. After 216 h, only 5.20, 5.83, and 6.85% of PHE was degraded in the 0.5% BC‐amended soils initially containing organic carbon at 0.23, 2.1, and 7.1%, respectively. Significant correlation was found between PHE degradability and freely dissolved concentration, suggesting that BC affected PHE bioavailability by decreasing chemical activity. The effect of activated carbon in the amended soils was attributed to its enhancement of soil surface areas and pore volumes. Results from the present study clearly highlighted the importance of BC for influencing the microbial availability of polycyclic aromatic hydrocarbons in soils.