The minimum size that a protein-free ADP-ribose chain requires to precipitate in 20% (w/v) trichloroacetic acid is 14 units.
The minimum size that a protein-free ADP-ribose chain requires to precipitate in 20% (w/v) trichloroacetic acid is 14 units.
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％20最小％20大小％20，％20a％20无蛋白质％20ADP-核糖％20链％20需要％20到％20沉淀％20在％2020％％20（w/v）％20三氯乙酸％20酸％20是％2014％20单位。
DOI：
10.1006/abio.1997.2115
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Alvarez-Gonzalez,R
中科院分区：
文献类型：
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作者：
Pacheco-Rodriguez,G;Alvarez-Gonzalez,R
以粗体显示的ATG表示编码NLS、B42 AD和Hg标签的下游序列所需的阅读框。如果利用NcoI或下游站点进行克隆，ATG - North Texas Health Science Center at Fort Worth， 3500包含在MCS的NcoI站点中，可以作为起始Camp Bowie Boulevard， Fort Worth， Texas 76107-2699蛋氨酸。pNB42载体包含与pJG4-5相同的可选择标记（6,7），即在酵母中选择TRP1，在细菌中选择AmpR。引物GTC-3和5- cgataaactgctcaatgatgg -3可用于从5和3方向跨MCS的DNA序列分析。
The ATG shown in bold type denotes the reading frame required for sequences downstream that encode the NLS, B42 AD, and Hg tag. Department of Microbiology and Immunology, University of If NcoI or sites downstream are utilized for cloning, the ATG con- North Texas Health Science Center at Fort Worth, 3500 tained in the NcoI site of the MCS can function as the initiation Camp Bowie Boulevard, Fort Worth, Texas 76107-2699 methionine. The pNB42 vectors contain the same selectable markers as pJG4-5 (6, 7), ie, TRP1 for selection in yeast and AmpR for selection in bacteria. The primers 5-GTATTACTTCTTATTCAAAT- Received December 9, 1996 GTC-3 and 5-CGATAAACTGCTCAATGATGG-3 can be used for DNA sequence analysis across the MCS from the 5 and 3 directions,