Effects of serum from rats with combined radiation-burn injury on the growth of hematopoietic progenitor cells
Effects of serum from rats with combined radiation-burn injury on the growth of hematopoietic progenitor cells
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DOI：
10.1097/01.ta.0000215434.24726.72
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发表时间：
2007-01-01
影响因子：
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通讯作者：
Cheng, Tian-Min
中科院分区：
文献类型：
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作者：
Ran, Xin-Ze;Su, Yong-Ping;Cheng, Tian-Min
背景：。本研究旨在观察放烧复合伤大鼠血清对造血祖细胞生长的影响，并探讨其可能的作用机制。方法：分别于伤后3小时、12小时、24小时、48小时、72小时、96小时采集放烧复合伤大鼠血清，加入培养基中观察终蛋白浓度为10微克/毫升时对造血祖细胞生长的影响。采用放射免疫法和酶联免疫吸附试验检测各组大鼠血清中肿瘤坏死因子(TNF)-α和白介素6(IL-6)水平，并观察其对UPC生长的影响。结果：烧伤或放烧复合伤大鼠加入血清后，HPC集落形成数在伤后3小时、12小时、24小时、48小时、72小时和96小时均明显高于正常大鼠，伤后24小时达到高峰。然而，加入照射后的大鼠血清后，发现HPC集落较少。同时，烧伤组和放烧复合伤组血清中的肿瘤坏死因子-α和IL-6水平均显著高于正常组，且显著高于放射损伤组(P<0.01)。结论：烧伤及放烧复合伤大鼠血清对HPC的生长有促进作用，而照射大鼠血清对HPC的生长有抑制作用。这些作用可能与各组血清中的肿瘤坏死因子-α和白介素6水平不同有关。
Background:. This study aims to observe the effects of blood serum from rats with radiation injury, burn injury, and combined radiation-burn injury on the growth of hematopoietic progenitor cells and to explore the possible mechanisms.Methods: Serum from rats with radiation injury, burn injury, and combined radiation-burn injury were collected at 3 hours, 12 hours, 24 hours, 48 hours, 72 hours, and 96 hours after injury and then was added to the culture medium to see its effect on the growth of hematopoietic progenitor cells (HPCs) at a final protein concentration of 10 mu g/mL. Radioimmunoassay and enzyme-linked immunosorbent assay were employed to measure the level of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 in each group, and the effect of TNF-alpha and IL-6 on the growth of UPC was also observed.Results: The number of HPCs colonies formed after addition of the serum from rats with burn or combined radiation-burn injuries was significantly higher than that from normal rats at 3 hours, 12 hours, 24 hours, 48 hours, 72 hours, and 96 hours after injury and reached its peak value at 24 hours after injury. However, fewer HPCs colonies were found after the addition of the serum from irradiated rats. At the same time, the levels of TNF-alpha and IL-6 in the serum of burn group and combined radiation-burn injury group were significantly higher than that of normal group, and much higher than that of the irradiation injury group (p < 0.01). Also, TNF-alpha and IL-6 demonstrated promoting effect on the growth of HPC.Conclusion: Serum from rats with burn injury and combined radiation-burn injury stimulates the growth of HPCs, while serum from irradiated rats shows inhibitory effects on the growth of HPCs. These effects may lie in the different level of TNF-alpha and IL-6 in the serum of each group.