Identification and characterization of Australian wild rice strains of Oryza meridionalis and Oryza rufipogon by SINE insertion polymorphism
Identification and characterization of Australian wild rice strains of Oryza meridionalis and Oryza rufipogon by SINE insertion polymorphism
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DOI：
10.1266/ggs.80.129
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发表时间：
2005-04-01
影响因子：
1.1
通讯作者：
Ohtsubo, E
中科院分区：
文献类型：
--
作者：
Xu, JH;Kurata, N;Ohtsubo, E
在具有AA基因组的水稻物种中，子午野生稻在澳大利亚北部被确定为一年生类型的物种，以前被归类为Oryza perennis、Oryza rufipogon或Oryza nivara。然而，这一概念导致了一些混乱，无法确定哪些菌株属于子午线稻瘟病菌，以及这些菌株与一年生类型的亚洲稻瘟病菌菌株的差异有多大。在这篇论文中，我们使用与每个p-SINE1成员两侧的序列杂交的引物，通过聚合酶链式反应检测了澳大利亚野生稻菌株是否存在p-SINE1成员，这些p-SINE1成员已被用于鉴定具有AA基因组的物种的品系。被检查的水稻品系包括多年生和一年生品系，以前被描述为O.rufipogon。我们发现，所有尚未确定类型的一年生菌株和其他菌株都具有在子午沙鼠标准菌株的相应座位上特异存在的p-SINE1成员，而在具有AA基因组的其他物种的菌株中没有这些特异存在于相应座位上的p-SINE1成员。然而，多年生菌株具有p-SINE1成员，这些成员专门存在于一年生或多年生类型的标准普通野生稻的相应基因座上，但没有那些特定存在于具有AA基因组的其他物种的菌株的相应基因座上的p-SINE1成员，包括南方野生稻。这些发现支持了先前的观点，即子午沙鼠是由一年生菌株组成的，是一个与普通沙鼠不同的物种。这项研究中使用的p-SINE1成员对于AA基因组物种的任何野生水稻品系的分类似乎非常有用，即使人们对水稻品系的形态、分类学、生理和生化了解有限。
Of the rice species with an AA genome, Oryza meridionalis has been identified in northern Australia as a species of the annual type, among those previously classified as Oryza perennis, Oryza rufipogon or Oryza nivara. This notion has, however, led to some confusion to determine which strains belong to O. meridionalis and how different these strains are from the O. rufipogon strains of the annual type. In this paper, we examined Australian wild rice strains for the presence or absence of p-SINE1 members, which have been used for identification of the strains of species with the AA genome, by PCR using primers that hybridize to the sequences flanking each p-SINE1 member. The rice strains examined include perennial and annual strains, which have previously been described as O. rufipogon. We found that all the annual strains and other strains, whose types have not been determined, have p-SINE1 members that are specifically present at the corresponding loci in the standard strains of O. meridionalis, but do not have those which are specifically present at the corresponding loci in the strains of the other species with the AA genome. The perennial strains, however, have p-SINE1 members that are specifically present at the corresponding loci in the standard O. rufipogon strains of either the annual or the perennial type, but do not have those which are specifically present at the corresponding loci in the strains of the other species with the AA genome, including O. meridionalis. These findings support the previous notion that O. meridionalis consists of the annual strains and is a distinct species from O. rufipogon. The p-SINE1 members used in this study appear to be very useful for classification of any wild rice strains of the AA-genome species, even when one has limited knowledge of morphology, taxonomy, physiology, and biochemistry of rice strains.