Magnetic activated cell sorting (MACS): utility in assisted reproduction.
Magnetic activated cell sorting (MACS): utility in assisted reproduction.
复制标题
磁激活细胞分选（MACS）：在辅助生殖中的实用性。
DOI：
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发表时间：
2008
影响因子：
0.6
通讯作者：
R. Sharma
中科院分区：
文献类型：
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作者：
K. Makker;A. Agarwal;R. Sharma
辅助生殖技术现已广泛纳入对不育夫妇的管理。但是，即使在方法和技术迅速进步之后，这些手术的成功率仍然低于预期。这导致了许多精子制备方案的发展，以获得理想的精液样本进行人工生殖。精子凋亡与生殖技术的失败密切相关。精子凋亡最早表现出的变化之一是磷脂酰丝氨酸的外化。磁激活细胞分选（MACS）是一种基于磷脂酰丝氨酸表达分离凋亡和非凋亡精子的新型精子制备技术。这导致了MACS作为精子制备技术的结合。综述了该技术的原理和作用机制，并列举了其作为精子制备技术的优势。它在抗逆转录病毒治疗中作为精子恢复的有效工具及其在精液样本冷冻保存中的应用也作了说明。
Assisted reproductive techniques (ART) have now been extensively incorporated in the management of infertile couples. But even after rapid methodological and technological advances the success rates of these procedures have been below expectations. This has led to development of many sperm preparation protocols to obtain an ideal semen sample for artificial reproduction. Sperm apoptosis has been heavily linked to failures in reproductive techniques. One of the earliest changes shown by apoptotic spermatozoa is externalization of phosphatidyl serine. Magnetic activated cell sorting (MACS) is a novel sperm preparation technique that separates apoptotic and non-apoptotic spermatozoa based on the expression of phosphatidylserine. This has led to the incorporation of MACS as a sperm preparation technique. The review highlights the principle and mechanism of this novel technique and enumerates its advantages as a sperm preparation technique. Its utility in ART as an efficient tool for sperm recovery and its application in cryopreservation of semen samples is also explained.