Genome-wide identification and analysis of the aldehyde dehydrogenase (ALDH) gene superfamily in apple (Malus x domestica Borkh.)
Genome-wide identification and analysis of the aldehyde dehydrogenase (ALDH) gene superfamily in apple (Malus x domestica Borkh.)
复制标题
苹果（Malus x Domestica Borkh.）醛脱氢酶（ALDH）基因超家族的全基因组鉴定和分析
DOI：
10.1016/j.plaphy.2013.07.017
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发表时间：
2013-10-01
影响因子：
6.5
通讯作者：
Wang, Xiping
中科院分区：
文献类型：
--
作者：
Li, Xiaoqin;Guo, Rongrong;Wang, Xiping
乙醛脱氢酶（ALDH）代表编码NAD（P）（+）依赖性酶的蛋白质超家族，所述酶氧化广泛范围的内源性和外源性脂肪族和芳香族醛。在植物中，它们参与许多生物过程，并在应对环境胁迫中发挥作用。本研究从苹果（Malus x arctica Borkh.）中鉴定了10个家系的39个ALDH基因。基因组苹果ALDH（MdALDH）基因的同线性分析表明，部分和串联重复以及全基因组重复可能是苹果ALDH基因家族扩展和进化的原因。此外，苹果和拟南芥之间的同线分析表明，几个MdALDH基因被发现在相应的同线块的拟南芥，这表明这些基因出现之前的谱系，导致苹果和拟南芥的分歧。此外，系统发育分析，以及外显子-内含子和蛋白质结构的比较，提供了进一步了解他们的进化关系和他们的假定功能。组织特异性表达分析的MdALDH基因表现出不同的时空表达模式，而在非生物胁迫和各种激素处理下的表达谱表明，许多MdALDH基因响应高盐和干旱，以及不同的植物激素。这种全基因组鉴定，以及表征苹果MdALDH基因的进化关系和表达谱，不仅有助于进一步分析ALDH基因及其在胁迫反应中的作用，而且还可能有助于未来苹果耐逆性的提高。(C)2013年Elsevier Masson SAS。All rights reserved.
Aldehyde dehydrogenases (ALDHs) represent a protein superfamily encoding NAD(P)(+)-dependent enzymes that oxidize a wide range of endogenous and exogenous aliphatic and aromatic aldehydes. In plants, they are involved in many biological processes and play a role in the response to environmental stress. In this study, a total of 39 ALDH genes from ten families were identified in the apple (Malus x domestica Borkh.) genome. Synteny analysis of the apple ALDH (MdALDH) genes indicated that segmental and tandem duplications, as well as whole genome duplications, have likely contributed to the expansion and evolution of these gene families in apple. Moreover, synteny analysis between apple and Arabidopsis demonstrated that several MdALDH genes were found in the corresponding syntenic blocks of Arabidopsis, suggesting that these genes appeared before the divergence of lineages that led to apple and Arabidopsis. In addition, phylogenetic analysis, as well as comparisons of exon-intron and protein structures, provided further insight into both their evolutionary relationships and their putative functions. Tissue-specific expression analysis of the MdALDH genes demonstrated diverse spatiotemporal expression patterns, while their expression profiles under abiotic stress and various hormone treatments indicated that many MdALDH genes were responsive to high salinity and drought, as well as different plant hormones. This genome-wide identification, as well as characterization of evolutionary relationships and expression profiles, of the apple MdALDH genes will not only be useful for the further analysis of ALDH genes and their roles in stress response, but may also aid in the future improvement of apple stress tolerance. (C) 2013 Elsevier Masson SAS. All rights reserved.