Inhibitory effects of Irigenin from the rhizomes of Belamcanda chinensis on nitric oxide and prostaglandin E2 production in murine macrophage RAW 264.7 cells
Inhibitory effects of Irigenin from the rhizomes of Belamcanda chinensis on nitric oxide and prostaglandin E2 production in murine macrophage RAW 264.7 cells
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DOI：
10.1016/j.lfs.2005.09.041
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发表时间：
2006-04-11
期刊：
影响因子：
6.1
通讯作者：
Jung, SH
中科院分区：
文献类型：
--
作者：
Ahn, KS;Noh, EJ;Jung, SH
本文研究了从射干根茎中提取的六种黄酮类化合物对RAW 264.7巨噬细胞的抗炎作用。结果表明，鸢尾苷元浓度依赖性地抑制脂多糖（LPS）诱导的一氧化氮（NO）和前列腺素（PG）E-2的产生。此外，该化合物抑制诱导型一氧化氮合酶（iNOS）和环氧合酶（考克斯）-2蛋白和mRNA的表达，而没有明显的细胞毒性作用。用鸢尾苷元处理转染的RAW 264.7细胞降低核因子-κ B（NF-κ B）活性的水平，也有效地降低电泳迁移率变动分析（EMSA）测量的NF-κ B结合，这与细胞核中p65蛋白水平的降低有关。以上结果提示，鸢尾苷元抑制LPS诱导的NO和PGE（2）合成的作用可能与抑制iNOS和考克斯-2的mRNA和蛋白表达有关，也可能与抑制NF-κ B B的活化有关。因此，从射干根茎中分离得到的鸢尾苷元可作为抗炎作用的先导化合物。(c)2005年爱思唯尔公司All rights reserved.
In the present study, we investigated anti inflammatory effects of six flavonoids isolated from the rhizomes of Belamcanda chinensis (Iridaceae) in RAW 264.7 macrophages. The results indicated that irigenin concentration dependently inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin (PG) E-2 production. Furthermore, this compound inhibited the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 proteins and mRNAs without an appreciable cytotoxic effect. Treatment of the transfectant RAW 264.7 cells with irigenin reduced the level of nuclear factor-kappa B (NF-kappa B) activity, also effectively lowered NF-kappa B binding measured by electrophoretic mobility shift assay (EMSA), which was associated with decreased p65 protein levels in the nucleus. On the basis of the above data, we suggest that the effect of irigenin in decreasing LPS-induced NO and PGE(2) synthesis is due to diminish the mRNA and protein expression of iNOS and COX-2, respectively, also may be due to under the suppression of NF-kappa B activation. Therefore, irigenin isolated from the rhizomes of Belamcanda chinensis could be offered as a leading compound for antiinflammation. (c) 2005 Elsevier Inc. All rights reserved.