Human malignant glioma cells express semaphorins and their receptors, neuropilins and plexins
Human malignant glioma cells express semaphorins and their receptors, neuropilins and plexins
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作者：
Rieger, J;Wick, W;Weller, M
信号蛋白包含一系列参与引导轴突和神经祖细胞生长的分子。此外，信号蛋白已被认为在癌症转移中发挥作用。 Neuropilins 1 和 2 是可溶性 3 类信号蛋白的细胞表面受体。丛蛋白是膜结合信号蛋白的直接受体，并且通过与神经毡蛋白结合，成为 3 类信号蛋白信号转导必需的辅助受体。我们在此报道人类恶性胶质瘤细胞系表达神经毡蛋白 1 和 2 mRNA 和蛋白，以及丛蛋白 A1、A2 或 B1。此外，所有神经胶质瘤细胞系都表达SEMA3A和SEMA3C并表现出SEMA3A结合位点。通过 F-肌动蛋白染色和胶原共培养测定确定，在 293 或 U87MG 细胞中表达的外源 SEMA3A 对神经胶质瘤细胞没有崩解或化学排斥活性。总之，人类神经胶质瘤细胞表达3类信号蛋白和可溶性和膜结合信号蛋白的受体，表明信号蛋白/神经毡蛋白系统在人类恶性神经胶质瘤与宿主中枢神经和免疫系统的相互作用中可能发挥作用。 (C) 2003 Wiley-Liss, Inc.
Semaphorins comprise a family of molecules implicated in the guiding of growing axons and neuronal progenitor cells. Further, semaphorins have been suggested to play a role in cancer metastasis. Neuropilins 1 and 2 are cell surface receptors for soluble class 3 semaphorins. Plexins are direct receptors for membrane-bound semaphorins and, by binding to neuropilins, coreceptors necessary for class 3 semaphorin signaling. We here report that human malignant glioma cell lines express neuropilins 1 and 2 mRNA and protein, as well as either plexin A1, A2, or B1. Further, all glioma cell lines express SEMA3A and SEMA3C and exhibit SEMA3A binding sites. Exogenous SEMA3A expressed in 293 or U87MG cells has no collapsing or chemorepulsive activities on glioma cells as determined by F-actin staining and collagen coculture assays. In summary, human glioma cells express class 3 semaphorins and receptors for soluble and membrane-bound semaphorins, suggesting a possible role of the semaphorin/neuropilin system in the interactions of human malignant glioma with the host's central nervous and immune systems. (C) 2003 Wiley-Liss, Inc.