Expression of transforming growth factor-beta type II receptor in rat lung is regulated during development.
Expression of transforming growth factor-beta type II receptor in rat lung is regulated during development.
复制标题
大鼠肺中转化生长因子-β II 型受体的表达在发育过程中受到调节。
DOI：
10.1152/ajplung.1995.269.3.l419
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Young,SL
中科院分区：
文献类型：
--
作者：
Zhao,Y;Young,SL
转化生长因子-β（TGF-β）是调节肺中细胞增殖、分化、细胞外基质产生和各种其它细胞功能的自分泌/旁分泌生长因子。TGF-β通过与跨膜异聚丝氨酸-苏氨酸激酶受体结合对细胞发挥作用。然而，尚未研究肺中特异性TGF-β受体的表达和定位。在本研究中，我们使用聚合酶链反应方法从大鼠胎肺中分离了一个1，762碱基对的cDNA，该cDNA包含TGF-β II型受体（T β RII）的全长编码序列。北方分析肺发育过程中T β RII的表达。在大鼠肺组织中检测到5.1-腺苷酸酶T β RII mRNA。T β RII mRNA在胎鼠肺组织发育早期即有表达，随发育进程而增加，出生后达高峰，随后下降至成年水平。用反义RNA探针进行原位杂交，研究了胎鼠和出生后大鼠肺组织中T β RII的定位。在妊娠第16天，T β RII基因在发育中的气道的间充质组织和上皮衬里中表达。在小血管外膜层也观察到T β RII mRNA的杂交信号。T β RII基因在发育中的气道上皮中的表达沿沿着近端-远端梯度发生。出生后肺组织中T β R Ⅱ mRNA主要表达于肺实质组织和血管中。T β RII在肺泡间隔的结缔组织中仍有高表达。（250字处删节）
Transforming growth factor-beta (TGF-beta) is an autocrine/paracrine growth factor that regulates cell proliferation, differentiation, extracellular matrix production and various other cell functions in the lung. TGF-beta exerts its effects on cells by binding to transmembrane heteromeric serine-threonine kinase receptors. The expression and localization of specific TGF-beta receptors in the lung, however, have not yet been investigated. In the present studies, we isolated a 1,762-base pair cDNA containing the full-length coding sequence for TGF-beta type II receptor (T beta RII) from rat fetal lung with the use of polymerase chain reaction methods. The expression of T beta RII during lung development was examined by Northern analysis. A 5.1-kilobase T beta RII mRNA was detected in rat lung tissue. T beta RII mRNA was expressed in rat fetal lung tissue early in development, increased as development proceeded, reached maximal concentration postnatally, and then decreased to the adult level. The localization of T beta RII in fetal and postnatal rat lung tissue was investigated with the use of in situ hybridization performed with an antisense RNA probe. T beta RII gene was expressed in the mesenchymal tissue and in the epithelial lining of the developing airway at day 16 of gestation. The hybridization signal of T beta RII mRNA was also observed in the adventitial layer of small blood vessels. Expression of T beta RII gene in the developing airway epithelium occurred along a proximal-distal gradient. In postnatal lung, T beta RII mRNA was detected mainly in parenchymal tissues and blood vessels. Expression of T beta RII remained high in the interstitium of interalveolar septa.(ABSTRACT TRUNCATED AT 250 WORDS)