Activin and activin receptor expression changes in liver regeneration in rat
Activin and activin receptor expression changes in liver regeneration in rat
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DOI：
10.1016/j.jss.2005.01.002
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发表时间：
2005-06-01
影响因子：
2.2
通讯作者：
Sugino, H
中科院分区：
文献类型：
--
作者：
Takamura, K;Tsuchida, K;Sugino, H
背景。本研究旨在通过比较70%肝部分切除（PH）和门静脉分支结扎（PBL）后大鼠残叶肝再生情况，探讨肝细胞增殖的调控机制。PH和PBL后检测活化素β A、β C和β E及其受体的表达。采用增殖细胞核抗原（PCNA）标记指数监测肝细胞增殖。PBL大鼠再生肺叶的PCNA标记指数在48 h达到峰值，比PH大鼠残叶延迟24 h。术后早期，激活素β A、β C和β E mRNA在PBL中的表达强于PH， 72 h时，激活素受体IIA型mRNA在PH中表达达到峰值，而在PBL中表达明显降低。肝细胞增殖、活化素及其受体的调控表达在PH和PBL后的大鼠肝脏再生过程中有所不同。因此，激活素信号通过受体的调节是决定PH和PBL后肝脏再生的因素之一。(C) 2005爱思唯尔公司版权所有。
Background. This study aimed to investigate regulatory mechanisms of hepatocyte proliferation by comparing liver regeneration of the remnant lobe after 70% partial hepatectomy (PH) and portal vein branch ligation (PBL) in rat.Methods. Expressions of activins beta A, beta C, and beta E and their receptors were investigated after PH and PBL. The proliferating cell nuclear antigen (PCNA) labeling index was used to monitor hepatocyte proliferation.Results. The PCNA labeling index in the regenerative lobe of PBL rats reached a peak at 48 h, a delay of 24 h compared with the remnant lobe in PH rats. In the postoperative early stage, the expression of activin beta A, beta C, and beta E mRNAs was stronger in PBL than PH. At 72 h the expression of activin receptor type IIA mRNA reached a peak in PH but was significantly lower in PBL.Conclusions. Hepatocyte proliferation, and the regulated expression of activins and their receptors, differs during liver regeneration after PH and PBL in the rat. Thus, regulation of activin signaling through receptors is one of the factors determining liver regeneration after PH and PBL. (C) 2005 Elsevier Inc. All rights reserved.