Genomic characterization of clinical Enterobacter roggenkampii co-harbouring blaIMP-1- and blaGES-5-encoding IncP6 and mcr-9-encoding IncHI2 plasmids isolated in Japan
Genomic characterization of clinical Enterobacter roggenkampii co-harbouring blaIMP-1- and blaGES-5-encoding IncP6 and mcr-9-encoding IncHI2 plasmids isolated in Japan
复制标题
DOI：
10.1016/j.jgar.2020.11.028
复制
发表时间：
2021-01-28
影响因子：
4.6
通讯作者：
Kawahara, Ryuji
中科院分区：
文献类型：
--
作者：
Umeda, Kaoru;Nakamura, Hiromi;Kawahara, Ryuji
目的：具有粘菌素耐药性的产碳青霉烯酶肠球菌（CPE）的传播是一个关键的公共卫生问题。我们对临床分离株罗氏肠杆菌OIPH-N260进行了遗传学表征，该菌株携带碳青霉烯酶基因bla（IMP-1）和bla（GES-5），并具有多个耐药基因，包括mcr-9和bla（CTX-M-9）。通过全基因组测序、耐药质粒比较分析、药敏试验、细菌接合试验、S1核酸酶消化脉冲场凝胶电泳，结果：OIPH-N260菌株对大多数β-内酰胺类抗生素和粘菌素耐药。它共携带两种耐药质粒，编码blaIMP-1和blaGES-5的IncP 6质粒pN 260 -3和编码mcr-9和bla（CTX-M-9）的IncHI（2）质粒pN 260 -1。对pN 260 -3的比较分析表明，在bla（GES-5）编码质粒中插入了一个独特的bla（IMP-1）周围区域，该区域具有移动的元件IS 26，在抗性基因的传播中起重要作用。pN 260 -1不含mcr-9表达调控基因qseBC。结论：本研究首次报道了在阴沟肠杆菌复合菌中不仅存在一个bla（IMP-1）和bla（GES-5）共编码质粒，而且还存在另一个携带mcr-9和bla的质粒（CTX-M-9）。通过IS 26介导的插入和耐药质粒的共携带而产生的晚期耐药突出了监测CPE中耐药基因的必要性。(C)2021作者由Elsevier Ltd代表国际抗微生物化疗学会发表。
Objectives: The spread of carbapenemase-producing Enterobacterales (CPE) with colistin resistance is a critical public health issue. We genetically characterized the clinical isolate Enterobacter roggenkampii OIPH-N260, which harboured carbapenemase genes bla(IMP-1) and bla(GES-5) with multiple resistance genes, including mcr-9 and bla(CTX-M-9).Methods: This isolate was characterized by whole-genome sequencing, comparative analysis of resistance plasmids, susceptibility tests, bacterial conjugation, S1-nuclease digested pulsed -field-gel electrophoresis, and Southern blot hybridization.Results: The OIPH-N260 isolate exhibited resistance to most beta-lactams and colistin. It co-harboured two resistance plasmids, the blaIMP-1- and blaGES-5-encoding IncP6 plasmid pN260-3 and mcr-9-and bla(CTX-M-9)-encoding IncHI(2) plasmid pN260-1. The comparative analysis of pN260-3 indicated that a unique bla(IMP-1)-surrounding region was inserted into the bla(GES-5)-encoding plasmid with the mobile element IS26, which plays an important role in the spread of resistance genes. pN260-1 did not possess the mcr-9 expression regulative gene qseBC. Both plasmids were transferable into other bacterial species via conjugation.Conclusions: This is the first study to report not only a bla(IMP-1) and bla(GES-5) co-encoding plasmid, but also the co-harbouring of another plasmid carrying mcr-9 and bla(CTX-M-9) in Enterobacter cloacae complex. The development of advanced resistance via IS26-mediated insertion and the co-harbouring of resistance plasmids highlights the need to monitor for resistance genes in CPE. (C) 2021 The Authors. Published by Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy.