Regional tumor oxygen dynamics: F-19 PBSR EPI of hexafluorobenzene
Regional tumor oxygen dynamics: F-19 PBSR EPI of hexafluorobenzene
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DOI：
10.1016/s0730-725x(97)00035-0
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发表时间：
1997-01-01
影响因子：
2.5
通讯作者：
Antich, PP
中科院分区：
文献类型：
--
作者：
Le, D;Mason, RP;Antich, PP
我们展示了一种新的方法来测量区域肿瘤氧分压使用F-19脉冲猝发饱和恢复回波平面成像（EPI）弛豫法的六氟苯。六氯苯对氧张力的变化具有特殊的敏感性，并且具有单一共振，使其成为成像研究的理想选择。通过将脉冲群饱和恢复准备序列与EPT相结合，在类似于20分钟的时间内进行弛豫实验，以便于测量伴随干预的pO（2）的动态变化，直接瘤内给予六氟苯可以标记特定的感兴趣区域，成像提供了pO（2）图，证实了不同的瘤内异质性，对于一组三个Dunning前列腺腺癌R3327-AT 1肿瘤，当大鼠呼吸33%O-2时，对中央肿瘤区域的询问显示出偏斜的pO（2）分布，具有相当大的放射生物学缺氧（类似于90%的体素具有pO（2）< 15 μ m）。在两个病例中，将吸入气体改为纯氧导致分布向pO（2）增加的方向移动，平均氧张力显著增加（p < 0.05）。第四个肿瘤的中心和外周区域的询问显示肿瘤氧合的双峰分布，包括类似于75%的pO（2）> 15 μ m的体素，EPI允许追踪单个体素的命运：在将吸入气体改变为纯氧时，基线pO（2）> 30 μ l的体素显示出显著变化（p < 0.05），而pO（2）< 16 μ g的患者则表现出最小的反应。测量的精度，以及同时检查多个区域的动态变化的能力，应该提供一种有用的技术，用于研究肿瘤缺氧治疗。(C)1997年爱思唯尔科学公司
We demonstrate a novel approach to measuring regional tumor oxygen tension using F-19 pulse burst saturation recovery echo planar imaging (EPI) relaxometry of hexafluorobenzene. Hexafluorobenzene offers exceptional sensitivity to changes in oxygen tension, and has a single resonance making it ideal for imaging studies, By combining a pulse burst saturation recovery preparation sequence with EPT, the relaxation experiments were performed in similar to 20 min facilitating measurements of dynamic changes in pO(2) accompanying interventions, Direct intratumoral administration of hexafluorobenzene permitted labeling of specific regions of interest, and imaging provided maps of pO(2), confirming distinct intra tumoral heterogeneity, For a group of three Dunning prostate adenocarcinoma R3327-AT1 tumors interrogation of the central tumor region showed skewed pO(2) distributions with considerable radiobiological hypoxia (similar to 90% voxels had pO(2) < 15 torr) when rats breathed 33% O-2. Altering the inspired gas to pure oxygen caused distributions to shift towards increased pO(2) with significant increases in mean oxygen tension (p < 0.05) in two cases. Interrogation of both central and peripheral regions in a fourth tumor showed bimodal distribution for tumor oxygenation including similar to 75% voxels with pO(2) > 15 torr, EPI allows the fate of individual voxels to be traced: upon altering the inspired gas to pure oxygen those voxels with baseline pO(2) > 30 torr showed significant changes (p < 0.05), whereas those with pO(2) < 16 torr showed minimal response. The precision of the measurements, together with the ability to simultaneously examine dynamic changes in multiple regions should provide a useful technique for investigating tumor hypoxia with respect to therapy. (C) 1997 Elsevier Science Inc.