Differential effects on innate versus adaptive immune responses by WF10
Differential effects on innate versus adaptive immune responses by WF10
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DOI：
10.1016/j.cellimm.2004.08.001
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发表时间：
2004-06-01
影响因子：
4.3
通讯作者：
Meuer, SC
中科院分区：
文献类型：
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作者：
Giese, T;McGrath, MS;Meuer, SC
体内生理产生的氧化化合物可以诱导自然防御机制，以增强病原体的清除，并在炎症过程中限制炎症组织的损伤。在此，我们研究了WF10，一种基于亚氯酸盐的无毒化合物，在体外对人PBMC的功能活性。WF10通过产生内源性氧化化合物，如牛磺酸氯胺，发挥强大的免疫调节作用。WE10抑制抗CD3刺激的PBMC的增殖和IL-2的产生，抑制转录因子NFATc的核转位。然而，在PBMC和单核细胞中，WF10诱导了IL-1β、IL-8和TNF-α等促炎细胞因子。在单核细胞系THP-1中，WF10可激活转录因子AP-1和NFkappaB。抑制活化淋巴细胞中NFAT调节的基因，同时诱导单核细胞中的几个髓系细胞相关的促炎基因，是一种新的免疫调节机制。(C)2004 Elsevier Inc.保留所有权利。
Oxidative compounds that are physiologically generated in vivo can induce natural defense mechanisms to enhance the elimination of pathogens and to limit inflammatory tissue damage in the course of inflammation. Here, we have investigated WF10, a chlorite-based non-toxic compound for its functional activities on human PBMC in vitro. WF10 exerts potent immune-modulatory effects through generating endogenous oxidative compounds such as taurine chloramine. Proliferation and IL-2 production of anti-CD3 stimulated PBMC were inhibited by WE 10, as was the nuclear translocation of the transcription factor NFATc. In PBMC and monocytes, however, WF10 induced pro-inflammatory cytokines like IL-1beta, IL-8, and TNF-alpha. In the monocytic cell line THP-1, the activation of the transcription factors AP-1 and NFkappaB by WF10 was demonstrated. Inhibition of NFAT regulated genes in activated lymphocytes in concert with the induction of several myeloid cell associated pro-inflammatory genes ill monocytes represents a novel mechanism of immune modulation. (C) 2004 Elsevier Inc. All rights reserved.