Intraspinal transplantation of mouse and human neural precursor cells.
Intraspinal transplantation of mouse and human neural precursor cells.
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小鼠和人类神经前体细胞的椎管内移植。
DOI：
10.1002/9780470151808.sc02d16s26
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发表时间：
2013
影响因子：
--
通讯作者：
Lane,ThomasE
中科院分区：
文献类型：
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作者：
Weinger,JasonG;Chen,Lu;Coleman,Ronald;Leang,Ronika;Plaisted,WarrenC;Loring,JeanneF;Lane,ThomasE
本单元描述了人神经前体细胞（hNPC）和小鼠神经前体细胞（mNPC）的制备和移植到小鼠脊髓的胸部区域。在这个单位的技术也描述了如何准备手术的小鼠进行椎板切除术暴露脊髓移植。在病毒介导的脱髓鞘后将用eGFP基因标记的NPC移植到小鼠脊髓中，可以通过eGFP表达有效地检测。将这些细胞移植到脊髓中是确定其在神经系统疾病如多发性硬化症、阿尔茨海默病和脊髓损伤中的作用的有效方法。方案干细胞生物学26：2D.16.1 - 2D.16.16。© 2013年由John Wiley & Sons，Inc.
This unit describes the preparation and transplantation of human neural precursor cells (hNPCs) and mouse neural precursor cells (mNPCs) into the thoracic region of the mouse spinal cord. The techniques in this unit also describe how to prepare the mouse for surgery by performing a laminectomy to expose the spinal cord for transplantation. NPCs genetically labeled with eGFP transplanted into the spinal cord of a mouse following viral‐mediated demyelination can efficiently be detected via eGFP expression. Transplantation of these cells into the spinal cord is an efficacious way to determine their effects in neurological disorders such as multiple sclerosis, Alzheimer's disease, and spinal cord injury.Curr. Protoc. Stem Cell Biol. 26:2D.16.1‐2D.16.16. © 2013 by John Wiley & Sons, Inc.