Adrenergic- and capsaicin-evoked nitric oxide release from urothelium and afferent nerves in urinary bladder
Adrenergic- and capsaicin-evoked nitric oxide release from urothelium and afferent nerves in urinary bladder
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DOI：
10.1152/ajprenal.1998.275.2.f226
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发表时间：
1998-08-01
影响因子：
4.2
通讯作者：
Kanai, AJ
中科院分区：
文献类型：
--
作者：
Birder, LA;Apodaca, G;Kanai, AJ
一氧化氮(NO)参与下尿路的调节。然而，膀胱(UB)中NO产生的来源(S)尚未确定。因此，我们在体外使用了放置在UB试条表面的卟啉微型传感器来直接测量内源性NO的产生。传入神经毒素辣椒素和混合α/β-肾上腺素能激动剂去甲肾上腺素(NE)均可引起一过性(S 1~3)NO释放(50 nm~1.4mM)。去神经支配UB后，肾上腺素能介导的释放不会减少，但选择性地切除粘膜后，肾上腺素能介导的释放就会消失。而去UB神经后，辣椒素(50-900 nM)引起的释放明显减少。这些结果表明，去甲肾上腺素从输尿管上皮细胞释放NO，辣椒素从输尿管上皮细胞和神经组织释放NO。鉴于NO可能调节其他组织的上皮完整性和功能的报道，激动剂调节UB中的结构性一氧化氮合酶活性可能为调节膀胱和尿路上皮功能提供一种新的机制。
Nitric oxide (NO) has been implicated in the regulation of the lower urinary tract. However, the source(s) of NO production in the urinary bladder (UB) has not been determined. Accordingly, we used a porphyrinic microsensor placed on the surface of UB strips in vitro to directly measure endogenous NO production. The afferent neurotoxin, capsaicin, and the mixed alpha/beta-adrenergic agonist, norepinephrine (NE), both evoked transient (1-3 s) NO release (range 50 nM to 1.4 mu M). Adrenergic-mediated release was not decreased following denervation of the UB but was abolished following selective removal of the mucosa. On the other hand, release evoked by capsaicin (range 50-900 nM) was significantly decreased after UB denervation. These data indicate that NE releases NO from UB epithelium, and capsaicin releases NO from epithelium as well as nervous tissue in the UB. In light of reports that NO may regulate epithelial integrity and function in other tissues, agonist regulation of a constitutive nitric oxide synthase activity in the UB may provide a novel mechanism for modulation of bladder and urothelial function.