Template-directed synthesis on oligodeoxycytidylate and polydeoxycytidylate templates.
Template-directed synthesis on oligodeoxycytidylate and polydeoxycytidylate templates.
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寡脱氧胞苷酸和聚脱氧胞苷酸模板的模板指导合成。
DOI：
10.1016/0022-2836(85)90091-9
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发表时间：
1985
影响因子：
5.6
通讯作者：
Orgel,LE
中科院分区：
文献类型：
--
作者：
Chen,CB;Inoue,T;Orgel,LE
寡脱氧胞苷酸和聚脱氧胞苷酸是鸟苷5′-（磷酸-2-甲基咪唑）聚合的有效模板。它们可以取代相应的核糖低聚物，而不会大大改变反应过程。由于脱氧核苷酸的寡聚体比核糖寡聚体更容易合成，如果这一发现被证明是普遍的，那么它将极大地促进含有两个或更多个碱基的寡聚体的模板性质的研究。寡脱氧胞苷酸盐促进寡鸟苷酸盐的合成，其长度比模板长一个残基，产率高，寡鸟苷酸盐的长度比模板长两倍。这些反应的时间过程和区域特异性表明，“滑动”和“双模板”是决定反应产物模式的重要因素。
Oligodeoxycytidylic acids and polydeoxycytidylic acid are effective templates for the polymerization of guanosine 5′-(phospho-2-methylimidazolide). They may be substituted for the corresponding ribo-oligomers without greatly changing the course of the reactions. Since oligomers of deoxynucleotides are much more easily synthesized than the ribo-oligomers, this finding, if it proves general, should greatly facilitate the study of the template properties of oligomers containing two or more bases.Oligodeoxycytidylates facilitate the synthesis of oligoguanylates up to one residue longer than the template in high yield, and oligoguanylates up to twice the length of the template in significant yield. The time-course and regiospecificity of these reactions suggest that “sliding” and “double-templating” are important factors in determining the pattern of reaction products.