[INVITED] Optical imaging and localization of prospective scattering labels smaller than a single protein
[INVITED] Optical imaging and localization of prospective scattering labels smaller than a single protein
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DOI：
10.1016/j.optlastec.2018.08.014
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发表时间：
2019-01-01
影响因子：
5
通讯作者：
Piliarik, Marek
中科院分区：
文献类型：
--
作者：
Holanova, Kristyna;Vala, Milan;Piliarik, Marek
生物物质的标记是实现衍射极限之外的动态过程的可视化和实时跟踪的关键技术。虽然基于荧光的定位显微镜的精度受到信号饱和度的限制，但新一代的小散射标记（如金纳米颗粒）使定位精度低于单个蛋白质的大小。然而，散射标签的大小也是最明显的障碍，因为它们通常明显大于所得到的定位精度。我们解决了这种不一致性，并证明了检测和精确定位的金纳米粒子的直径只有2 nm的散射干涉检测（iSCAT）。这种尺寸比小蛋白质的尺寸小得多，并且结合跟踪纳米颗粒位置的可能性，我们预见了未来生物分子系统详细研究的新概念。
Labeling of biological matter is a key technique enabling visualization and real-time tracking of dynamic processes beyond the diffraction limit. While the precision of fluorescence-based localization microscopy is limited by signal saturation, a new generation of small scattering labels (such as gold nanoparticles) have brought the localization precision below the size of a single protein. However, the size of scattering labels is also the most obvious handicap, as they are usually significantly larger than the resulting localization precision. We address this inconsistency and demonstrate detection and precise localization of gold nanoparticles of only 2 nm in diameter using interferometric detection of scattering (iSCAT). This size is considerably smaller than the size of a small protein and in combination with the possibility of tracking the nanoparticle position, we foresee a new concept of detailed studies of biomolecular systems in the future.