Physico-Mechanical Properties of HA/TCP Pellets and Their Three-Dimensional Biological Evaluation In Vitro
Physico-Mechanical Properties of HA/TCP Pellets and Their Three-Dimensional Biological Evaluation In Vitro
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DOI：
10.1007/5584_2017_130
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发表时间：
2019-01-01
影响因子：
3.6
通讯作者：
Yahaya, Badrul Hisham
中科院分区：
文献类型：
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作者：
Kamalaldin, Nurulain 'Atikah;Jaafar, Mariatti;Yahaya, Badrul Hisham
生物陶瓷，特别是羟基磷灰石(HA)和β-磷酸三钙(β-TCP)作为三维支架在骨工程中的应用是必不可少的，因为这些元素加在一起构成了骨含量的60%。不同比例的羟基磷灰石和β-磷酸三钙先前已经测试了它们生产合适的生物陶瓷支架的能力，这种支架必须能够承受高机械负荷。在这项研究中，两种HA/TCP比例(20：80和70：30)被用来制作微丸，然后在体外进行评估，以确定在植骨中使用该材料的任何不良反应。通过径向拉伸强度(DTS)和密度测试来评估微丸的机械强度和孔隙率。然后测试了这些小球对正常人类成纤维细胞的毒性。在毒性实验中，细胞与微丸共同孵育3d。在实验结束时，评估细胞的形态变化，并使用PrestoBlue细胞活力试剂(TM)读取吸光度。DTS与孔隙率成反比关系。两种处理组成纤维细胞均显示正常细胞形态，说明HA/TCP微丸无毒。在成骨细胞附着实验中，细胞能够附着在两种比例的支架表面，但细胞也能够穿透到70：30的支架内。这一发现表明，70：30的比例比20：80的比例具有更好的骨传导性能。
The use of bioceramics, especially the combination of hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP), as a three-dimensional scaffold in bone engineering is essential because together these elements constitute 60% of the bone content. Different ratios of HA and beta-TCP were previously tested for their ability to produce suitable bioceramic scaffolds, which must be able to withstand high mechanical load. In this study, two ratios of HA/TCP (20:80 and 70:30) were used to create pellets, which then were evaluated in vitro to identify any adverse effects of using the material in bone grafting. Diametral tensile strength (DTS) and density testing was conducted to assess the mechanical strength and porosity of the pellets. The pellets then were tested for their toxicity to normal human fibroblast cells. In the toxicity assay, cells were incubated with the pellets for 3 days. At the end of the experiment, cell morphological changes were assessed, and the absorbance was read using PrestoBlue Cell Viability Reagent(TM). An inversely proportional relationship between DTS and porosity percentage was detected. Fibroblasts showed normal cell morphology in both treatments, which suggests that the HA/TCP pellets were not toxic. In the osteoblast cell attachment assay, cells were able to attach to the surface of both ratios, but cells were also able to penetrate inside the scaffold of the 70:30 pellets. This finding suggests that the 70:30 ratio had better osteoconduction properties than the 20:80 ratio.