Floral Transcriptomes in Woodland Strawberry Uncover Developing Receptacle and Anther Gene Networks
Floral Transcriptomes in Woodland Strawberry Uncover Developing Receptacle and Anther Gene Networks
复制标题
DOI：
10.1104/pp.114.237529
复制
发表时间：
2014-07-01
期刊：
影响因子：
7.4
通讯作者：
Liu, Zhongchi
中科院分区：
文献类型：
--
作者：
Hollender, Courtney A.;Kang, Chunying;Liu, Zhongchi
花是生殖器官，是果实和种子的前体。虽然花发育的ABCE模型的基本原则在被子植物中是保守的，但不同的开花植物表现出不同的，有时是独特的特征。草莓（Fragaria spp.）花是几百个独立的离生心皮（不融合）的发展。这些心皮在花托上呈螺旋状排列。因此，花托是草莓花的组成部分，并且具有重要的农艺学重要性，是草莓果实的前体。利用新一代测序技术和激光捕获显微切割技术，我们建立了林地草莓（Fragaria vesca）花发育不同组织和阶段的转录组学图谱。利用成对比较和加权基因共表达网络分析，我们确定了组织特异性网络的共表达基因和枢纽基因的模块。特别重要的是发现一个发展中的受体特异性模块表现出类似的分子特征的年轻的花分生组织。一些分生组织调节因子，包括LOST MERISTEM和WUSCHEL的草莓同源物，被确定为在发育中的花托网络中运作的枢纽基因。此外，基因组中几乎25%的F-box基因在发育中的花药中在减数分裂阶段被瞬时诱导，表明活性蛋白降解。总之，这项工作为草莓独特的生殖发育过程的分子网络提供了重要的见解。这个广泛的花转录组数据集是公开的，可以很容易地在项目网站上查询，作为一个重要的基因组资源的植物生物学研究社区。
Flowers are reproductive organs and precursors to fruits and seeds. While the basic tenets of the ABCE model of flower development are conserved in angiosperms, different flowering plants exhibit different and sometimes unique characteristics. A distinct feature of strawberry (Fragaria spp.) flowers is the development of several hundreds of individual apocarpous (unfused) carpels. These individual carpels are arranged in a spiral pattern on the subtending stem tip, the receptacle. Therefore, the receptacle is an integral part of the strawberry flower and is of significant agronomic importance, being the precursor to strawberry fruit. Taking advantage of next-generation sequencing and laser capture microdissection, we generated different tissue-and stage-transcriptomic profiling of woodland strawberry (Fragaria vesca) flower development. Using pairwise comparisons and weighted gene coexpression network analysis, we identified modules of coexpressed genes and hub genes of tissue-specific networks. Of particular importance is the discovery of a developing receptacle-specific module exhibiting similar molecular features to those of young floral meristems. The strawberry homologs of a number of meristem regulators, including LOST MERISTEM and WUSCHEL, are identified as hub genes operating in the developing receptacle network. Furthermore, almost 25% of the F-box genes in the genome are transiently induced in developing anthers at the meiosis stage, indicating active protein degradation. Together, this work provides important insights into the molecular networks underlying strawberry's unique reproductive developmental processes. This extensive floral transcriptome data set is publicly available and can be readily queried at the project Web site, serving as an important genomic resource for the plant biology research community.