An inexpensive "do-it-yourself" device for rapid generation of uniform tumor spheroids.
An inexpensive "do-it-yourself" device for rapid generation of uniform tumor spheroids.
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DOI：
10.1016/j.device.2024.100255
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发表时间：
2024-01
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影响因子：
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通讯作者：
Bumseok Namgung;Hongqing Dai;P. Vikraman;Tanmoy Saha;Shiladitya Sengupta;Hae Lin Jang
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文献类型：
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作者：
Bumseok Namgung;Hongqing Dai;P. Vikraman;Tanmoy Saha;Shiladitya Sengupta;Hae Lin Jang
三维（3D）癌细胞培养模型，如肿瘤球状体，比传统的二维模型更好地重现体内肿瘤。然而，两个主要的挑战限制了3D肿瘤球体的常规使用。首先，大多数现有的产生肿瘤球状体的方法不是高通量的。其次，使用当前方法生成的肿瘤球体在尺寸上高度可变。在这里，我们描述了一个简单的“自己动手”的设备，可以组装不到7美元的部分，可以产生均匀的肿瘤球状体在一个高通量的方式。我们使用了一个简单的电话硬币振动电机来消除振动，以将载有细胞的藻酸盐溶液的层流射流破碎成相等大小的球形珠。我们每分钟产生3，970个肿瘤球体，其在体内肿瘤中表现出缺氧核心重现，可用于测试抗癌药物的扩散效力。这种低成本、易于制造、操作简单、高通量的系统对于癌症研究的民主化和标准化至关重要。
Three-dimensional (3D) cancer cell culture models such as tumor spheroids better recapitulatein vivotumors than conventional two-dimensional models. However, two major challenges limit the routine use of 3D tumor spheroids. Firstly, most existing methods of generating tumor spheroids are not high throughput. Secondly, tumor spheroids generated using current methods are highly variable in dimension. Here, we describe a simple "do-it-yourself" device that can be assembled for less than $7 of parts that can generate uniform tumor spheroids in a high-throughput manner. We used a simple phone coin-vibrating motor to superimpose the vibration for breaking a laminar jet of cell-loaded alginate solution into equally sized spherical beads. We generated 3,970 tumor spheroids/min, which exhibited a hypoxic core recapitulatingin vivotumors and could be used to test the diffusion efficacy of anti-cancer drugs. Such low-cost, easy-to-fabricate, simple-to-operate systems with high-throughput outcomes are essential to democratize and standardize cancer research.