Transcriptome Landscape of Human Folliculogenesis Reveals Oocyte and Granulosa Cell Interactions
Transcriptome Landscape of Human Folliculogenesis Reveals Oocyte and Granulosa Cell Interactions
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人类卵泡发生的转录组景观揭示了卵母细胞和颗粒细胞的相互作用
DOI：
10.1016/j.molcel.2018.10.029
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发表时间：
2018-12-20
期刊：
影响因子：
16
通讯作者：
Yan, Liying
中科院分区：
文献类型：
--
作者：
Zhang, Yaoyao;Yan, Zhiqiang;Yan, Liying
在卵泡发生过程中，人类生殖系和周围体细胞的动态转录调控和相互作用尚不清楚。通过RNA测序（RNA-seq）分析跨越五个卵泡阶段的人类卵母细胞和相应的颗粒细胞（GCs），我们揭示了人类卵母细胞和GCs在转录机制、转录因子网络和相互作用方面的独特特征，这些特征显示出发育阶段特异性表达模式。值得注意的是，我们发现了两种细胞类型在特定发育阶段的特定基因特征，这些特征可能反映了发育能力和卵巢储备。此外，我们发现了可能协调生殖-体细胞相互作用并驱动原始卵泡向初级卵泡转变的关键途径，这代表了卵泡激活。因此，我们的工作为逐步卵泡形成中转录调控的关键特征提供了关键见解，并为改善体内卵泡募集和体外恢复完全胜任的卵母细胞提供了重要线索。
The dynamic transcriptional regulation and interactions of human germlines and surrounding somatic cells during folliculogenesis remain unknown. Using RNA sequencing (RNA-seq) analysis of human oocytes and corresponding granulosa cells (GCs) spanning five follicular stages, we revealed unique features in transcriptional machinery, transcription factor networks, and reciprocal interactions in human oocytes and GCs that displayed developmental- stage-specific expression patterns. Notably, we identified specific gene signatures of two cell types in particular developmental stage that may reflect developmental competency and ovarian reserve. Additionally, we uncovered key pathways that may concert germline-somatic interactions and drive the transition of primordial-to-primary follicle, which represents follicle activation. Thus, our work provides key insights into the crucial features of the transcriptional regulation in the stepwise folliculogenesis and offers important clues for improving follicle recruitment in vivo and restoring fully competent oocytes in vitro.