Catalpol ameliorates Sjogren's Syndrome by modulating interplay of T and B cells
Catalpol ameliorates Sjogren's Syndrome by modulating interplay of T and B cells
复制标题
梓醇通过调节 T 细胞和 B 细胞的相互作用来改善干燥综合症
DOI：
10.1016/j.biopha.2019.109806
复制
发表时间：
2020-03-01
影响因子：
7.5
通讯作者：
Wang, Yue
中科院分区：
文献类型：
--
作者：
Lin, Rui;Hao, Donglin;Wang, Yue
梓醇是治疗原发性干燥综合征（pSS）的常用草药黄芪的有效成分。然而，没有研究评估梓醇在治疗干燥综合征中的治疗效果。本研究旨在通过评估饮水量、刺激唾液流率、涎腺炎、T细胞亚群和相关细胞因子水平来研究梓醇对pSS的治疗作用。梓醇处理的小鼠与来自对照组的小鼠相比具有改善的刺激唾液流速和水消耗。梓醇还减少了淋巴细胞浸润，并防止异位生发中心的形成。这些效应，尤其是在梓醇高剂量组中，与升高的CD 4（+）CXC-R5（+）PD-1（+）Foxp 3（+）T滤泡调节（Tfr）细胞（1.572% vs 1.118%，P = 0.0005）和较高的Tfr细胞与T滤泡辅助（Tfh）细胞的比率（2.137 vs 1.541，P = 0.0007）相关。在梓醇处理组中还观察到血清和下颌下腺中IFN-γ和BAFF水平下调。我们的工作表明梓醇可能通过调节T和B细胞之间的相互作用而成为治疗pSS的潜在药物。
Catalpol is an active ingredient of Rehmanniae Radix, a medical herb used frequently in treating primary Sjogren's Syndrome (pSS). However, no study has assessed the therapeutic effects of catalpol in treating Sjogren's Syndrome. This study aimed to investigate the therapeutic effects of catalpol for pSS by evaluating the water consumption, stimulated salivary flow rates, sialadenitis, T cell subsets and related cytokine levels. Catalpol treated mice had improved stimulated salivary flow rates and water consumption compared to mice from the control group. Catalpol also reduced the lymphocytic infiltration and prevented the formation of ectopic germinal centers. These effects, especially in the catalpol high dose group, were associated with elevated CD4(+) CXC-R5(+) PD-1(+) Foxp3(+) T follicular regulatory (Tfr) cells (1.572 % vs 1.118 %, P = 0.0005) and a higher ratio of Tfr cells to T follicular helper (Tfh) cells (2.137 vs 1.541, P = 0.0007). Downregulated levels of IFN-gamma and BAFF in serum and submandibular glands were also noted in catalpol treated groups. Our work indicated that catalpol might be a potential drug for treating pSS by regulating the interplay between T and B cells.