Melghirimyces algeriensis gen. nov., sp nov., a member of the family Thermoactinomycetaceae, isolated from a salt lake
Melghirimyces algeriensis gen. nov., sp nov., a member of the family Thermoactinomycetaceae, isolated from a salt lake
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DOI：
10.1099/ijs.0.028985-0
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发表时间：
2012-07-01
影响因子：
2.8
通讯作者：
Fardeau, Marie-Laure
中科院分区：
文献类型：
--
作者：
Addou, Ammara Nariman;Schumann, Peter;Fardeau, Marie-Laure
从位于阿尔及利亚东南部的 Chott Melghir 盐湖收集的土壤中分离出一种新型丝状细菌，命名为 NariEX(T)。该菌株是一种需氧、耐盐、耐热的革兰氏阳性细菌，能够在 NaCl 浓度高达 21% (w/v)、37-60 摄氏度和 pH 5.0-9.5 的条件下生长。主要脂肪酸是异-和反异-C-15:0。 DNA G+C含量为47.3mol%。主要的甲基萘醌是 MK-7，但也存在 MK-6 和 MK-8。极性脂质谱由磷脂酰甘油、二磷脂酰甘油、磷脂酰乙醇胺和磷脂酰单甲基乙醇胺（甲基-PE）组成。分子和表型分析的结果将该菌株描述为嗜热放线菌科的新成员。该分离株在形态、生化和化学分类学特征上与该科公认属的成员不同。菌株NariEX(T)与典型菌株Desmospora activa和Kroppenstedtia eburnea的16S rRNA基因序列相似性分别为95.38%和94.28%，但在糖、极性脂质和甲基-PE的存在方面与两种典型菌株不同。根据生理和系统发育数据，菌株NariEX(T)代表了Thermoactinomycetaceae科新属的新种，其名称为Melghirimyces algeriensis gen。十一月，SP。十一月被提议。该属的模式种Melghirimyces algeriensis的模式菌株是NariEX(T)(＝DSM 45474(T)＝CCUG 59620(T))。
A novel filamentous bacterium, designated NariEX(T), was isolated from soil collected from Chott Melghir salt lake, which is located in the south-east of Algeria. The strain was an aerobic, halotolerant, thermotolerant, Gram-positive bacterium that was able to grow in NaCl concentrations up to 21 % (w/v), at 37-60 degrees C and at pH 5.0-9.5. The major fatty acids were iso- and anteiso-C-15:0. The DNA G + C content was 47.3 mol%. The major menaquinone was MK-7, but MK-6 and MK-8 were also present. The polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine (methyl-PE). Results of molecular and phenotypic analysis led to the description of the strain as a new member of the family Thermoactinomycetaceae. The isolate was distinct from members of recognized genera of this family by morphological, biochemical and chemotaxonomic characteristics. Strain NariEX(T) showed 16S rRNA gene sequence similarities of 95.38 and 94.28% with the type strains of Desmospora activa and Kroppenstedtia eburnea, respectively, but differed from both type strains in its sugars, polar lipids and in the presence of methyl-PE. On the basis of physiological and phylogenetic data, strain NariEX(T) represents a novel species of a new genus of the family Thermoactinomycetaceae for which the name Melghirimyces algeriensis gen. nov., sp. nov. is proposed. The type strain of Melghirimyces algeriensis, the type species of the genus, is NariEX(T) (=DSM 45474(T)=CCUG 59620(T)).