Parallel changes in serotonin levels in brain and blood following acute administration of MDMA
Parallel changes in serotonin levels in brain and blood following acute administration of MDMA
复制标题
DOI：
10.1177/0269881112463123
复制
发表时间：
2013-01-01
影响因子：
4.1
通讯作者：
Elliott, J. Martin
中科院分区：
文献类型：
--
作者：
Collins, Cheryl M.;Kloek, Joris;Elliott, J. Martin
最近的研究表明，3，4-亚甲基二氧基甲基苯丙胺(MDMA)通过作用于5-羟色胺转运体，在血液、血小板和脑组织中具有类似的急性作用。在这方面，为了研究血中5-羟色胺作为中枢5-羟色胺外周标志物的有效性，我们给予MDMA(20 mg/kg ip)。额叶皮质和血液中的5-羟色胺水平在2 h时下降(分别为63%和46%)，8 h时有所恢复(42%和38%)，18 h时更明显(分别比对照水平低19%和24%)。给予色氨酸补充剂(82.5 mg/kg P.O.)NA<；VE大鼠2小时后额叶皮质(39%)和血液(26%)的5-羟色胺水平也随之升高。在服用MDMA后，相同剂量的色氨酸导致脑内5-羟色胺的上升幅度较小(26%)，而在血液中则没有影响。我们的结论是，血液5-羟色胺是急性给药后大鼠脑内5-羟色胺水平的有用标记物，这一发现突出了在涉及MDMA的人类研究中使用血小板5-羟色胺作为脑5-羟色胺标记物的可能性。
Recent studies have demonstrated a similar acute effect of 3,4- methylenedioxymethamphetamine (MDMA) in blood platelets and brain tissue via action on the serotonin transporter. To investigate the validity of blood serotonin as a peripheral marker for central serotonin in this regard, we administered MDMA (20 mg/kg i.p.) to rats and observed a parallel decrease in serotonin levels in the frontal cortex and blood at 2 h (63% and 46% respectively) with some recovery evident at 8 h (42% and 38%) and more so at 18 h (19% and 24% below control levels). Administration of a tryptophan supplement (82.5 mg/kg p.o.) to na < ve rats produced parallel increases in serotonin levels 2 h later in the frontal cortex (39%) and blood (26%). Following MDMA administration, the same dose of tryptophan caused a smaller (26%) rise in brain serotonin whereas in blood it had no effect. We conclude that blood serotonin is a useful marker for brain serotonin levels in the rat following acute administration of MDMA and this finding highlights the possible use of platelet serotonin as a marker for brain serotonin in human studies involving MDMA.