Inhibition of de novo synthesis of FSH by the Sertoli cell factor (SCF).
Inhibition of de novo synthesis of FSH by the Sertoli cell factor (SCF).
复制标题
支持细胞因子 (SCF) 抑制 FSH 从头合成。
DOI：
10.1210/endo-103-2-644
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发表时间：
1978
期刊：
影响因子：
4.8
通讯作者：
E. Steinberger
中科院分区：
文献类型：
--
作者：
M. Chowdhury;A. Steinberger;E. Steinberger
本文研究了离体培养的大鼠睾丸支持细胞分泌的SCF对FSH和LH合成的影响。取成年雄性大鼠垂体前叶，在含SCF和不含SCF的培养液中培养24- 48 h。3 H-亮氨酸掺入FSH和LH测定后6小时孵育与此标记的前体和免疫沉淀与特定的FSH或LH抗体。用放射免疫法测定总FSH和LH。结果表明，SCF可选择性地减少~ 3 H-亮氨酸掺入免疫沉淀FSH，而不减少掺入LH。
The effects of SCF, a substance produced by isolated rat Sertoli cells in culture, on the synthesis of FSH and LH were studied in vitro. Anterior pituitaries from adult male rats were organ-cultured 24-48h in the presence and absence of SCF in the culture medium. The incorporation of 3H-leucine into FSH and LH was determined following 6h incubation with this labelled precursor and immunoprecipitation with specific FSH or LH antibodies. Total FSH and LH were measured by radioimmunoassay. The results indicate that SCF can selectively reduce the incorporation of 3H-leucine into immunoprecipitable FSH without decreasing the incorporation into LH.