A latex agglutination test using a recombinant nucleoprotein for detection of antibodies against avian influenza virus
A latex agglutination test using a recombinant nucleoprotein for detection of antibodies against avian influenza virus
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DOI：
10.1016/j.jviromet.2009.06.021
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发表时间：
2009-11-01
影响因子：
3.1
通讯作者：
Imai, K.
中科院分区：
文献类型：
--
作者：
Horie, M.;Ogawa, H.;Imai, K.
建立了检测禽流感病毒抗体的乳胶凝集试验（LAT）。将在大肠杆菌中表达的重组禽流感病毒核蛋白纯化，与乳胶珠偶联，并用作LAT的抗原。LAT能够检测抗禽流感病毒抗体，无论禽流感亚型，在大多数情况下，结果与琼脂凝胶沉淀试验（AGPT）的结果相关。但是，与AGPT相比，LAT可以在感染后较长时间内检测到抗禽流感病毒抗体。用干鸡肝粉预处理未感染鸡血清1小时，可消除未感染鸡血清中观察到的非特异性凝集。LAT易于进行，即使考虑到血清预处理所需的时间，与AGPT的情况下所需的时间相比，获得结果所需的总时间也减少了。这种简便、快速的LAT被认为对现场监测禽流感病毒感染是有用的。(C)2009爱思唯尔有限公司版权所有。
A latex agglutination test (LAT) was developed for detecting antibodies against avian influenza virus. The recombinant avian influenza virus nucleoprotein expressed in Escherichia coli was purified, coupled with latex beads, and used as an antigen for the LAT. The LAT was capable of detecting anti-avian influenza virus antibodies irrespective of the avian-influenza subtype, and in most cases, the results correlated with the results of an agar gel precipitation test (AGPT). However, in comparison with the AGPT the LAT could detect the anti-avian influenza virus antibodies for a longer period of time after the infection. The nonspecific agglutination observed in uninfected chicken sera was resolved by pretreating the sera with dried chicken-liver powder for I h. The LAT is easy to perform, and even after considering the time required for pretreatment of the serum, the total time required for obtaining the results is reduced in comparison to the time required in the case of the AGPT. This easy and rapid LAT is considered to be useful for monitoring avian influenza virus infection in the field. (C) 2009 Elsevier B.V. All rights reserved.