Transthoracic echocardiography in mice.
Transthoracic echocardiography in mice.
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DOI：
10.3791/1738
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发表时间：
2010-05-28
期刊：
影响因子：
--
通讯作者：
Wehrens, Xander H T
中科院分区：
文献类型：
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作者：
Respress, Jonathan L;Wehrens, Xander H T
近年来，小鼠模型已成为研究基因表达变化引起的心功能不全的分子机制的主要途径。转基因和基因靶向方法可用于产生心脏大小和功能改变的小鼠，因此，需要体内技术来评估其心脏表型。经胸超声心动图、脉冲波多普勒（PWD）和组织多普勒成像（TDI）可用于提供小鼠心脏的尺寸测量并量化心脏收缩和舒张性能的程度。二维成像用于检测左心室的异常解剖结构或运动，而M模式回波用于量化心脏尺寸和收缩力。此外，PWD用于量化湍流的局部速度，而TDI用于测量心肌运动的速度。因此，经胸超声心动图提供了一个全面的方法，在小鼠心脏功能的非侵入性评价。
In recent years, murine models have become the primary avenue for studying the molecular mechanisms of cardiac dysfunction resulting from changes in gene expression. Transgenic and gene targeting methods can be used to generate mice with altered cardiac size and function, and as a result, in vivo techniques are needed to evaluate their cardiac phenotype. Transthoracic echocardiography, pulse wave Doppler (PWD), and tissue Doppler imaging (TDI) can be used to provide dimensional measurements of the mouse heart and to quantify the degree of cardiac systolic and diastolic performance. Two-dimensional imaging is used to detect abnormal anatomy or movements of the left ventricle, whereas M-mode echo is used for quantification of cardiac dimensions and contractility. In addition, PWD is used to quantify localized velocity of turbulent flow, whereas TDI is used to measure the velocity of myocardial motion. Thus, transthoracic echocardiography offers a comprehensive method for the noninvasive evaluation of cardiac function in mice.