Intravital Microscopy in Atherosclerosis Research.
Intravital Microscopy in Atherosclerosis Research.
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DOI：
10.1007/978-1-0716-1924-7_40
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发表时间：
2022
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动脉粥样硬化是一种脂质驱动的炎症性疾病，可使动脉管腔变窄，并可诱发冠状动脉疾病、脑血管疾病和外周动脉疾病等危及生命的并发症。在机械水平上，新的细胞分辨率活体显微镜成像方法的发展，最近使在体内研究的基础生物过程中，疾病的发病和进展。特别是多光子显微镜已经成为一个有前途的活体成像工具，利用双光子激发荧光和二次谐波产生，提供亚细胞分辨率和增加的成像深度超越共聚焦和落射荧光显微镜。在这一章中，我们描述了国家的最先进的多光子显微镜应用于小鼠动脉粥样硬化的研究。
Atherosclerosis is a lipid-driven inflammatory disorder that narrows the arterial lumen and can induce life-threatening complications from coronary artery disease, cerebrovascular disease and peripheral artery disease. On a mechanistic level, the development of novel cellular-resolution intravital microscopy imaging approaches has recently enabled in vivo studies of underlying biological processes governing disease onset and progress. In particular multi-photon microscopy has emerged as a promising intravital imaging tool utilizing two-photon-excited fluorescence and second-harmonic generation that provides sub-cellular resolution and increased imaging depths beyond confocal and epifluorescence microscopy. In this chapter, we describe the state-of-the-art multi-photon microscopy applied to the study of murine atherosclerosis.