In situ synthesis of peptide nucleic acids in porous silicon for drug delivery and biosensing.
In situ synthesis of peptide nucleic acids in porous silicon for drug delivery and biosensing.
复制标题
DOI：
10.1021/bc5001092
复制
发表时间：
2014-07-16
影响因子：
4.7
通讯作者：
Duvall, Craig L.
中科院分区：
文献类型：
--
作者：
Beavers, Kelsey R.;Mares, Jeremy W.;Swartz, Caleb M.;Zhao, Yiliang;Weiss, Sharon M.;Duvall, Craig L.
肽核酸（PNA）是一类独特的合成分子，其具有肽骨架并且可以与核酸杂交。在这里，一个通用的方法已经开发的自动化，在原位合成的PNA从多孔硅（PSi）基板在基因治疗和生物传感的应用。进行非破坏性的光学测量，以监测从（3-氨丙基）三乙氧基硅烷连接到PSi膜的表面发起的PNA的单碱基添加，并进行质谱分析，以验证所需序列的合成。比较原位合成合成后的表面共轭的完整PNA分子表明，表面介导的，原位PNA合成增加加载8倍。对于治疗性概念验证，在磷酸盐缓冲盐水中表征来自PSi膜的受控PNA释放，并且由含有与微RNA（miR）122互补的原位生长的PNA的PSi膜制造的PSi纳米颗粒在Huh 7 psiRNAK-miR 122细胞系中产生显著的抗miR活性。该平台的生物传感的适用性也证明了使用光学测量，表明选择性杂交互补DNA靶分子的PNA原位合成的PSi膜。这些集体数据证实，我们已经建立了一种新的PNA-PSi平台，在药物递送和生物传感中具有广泛的用途。
Peptide nucleic acids (PNA) are a unique class of synthetic molecules that have a peptide backbone and can hybridize with nucleic acids. Here, a versatile method has been developed for the automated, in situ synthesis of PNA from a porous silicon (PSi) substrate for applications in gene therapy and biosensing. Nondestructive optical measurements were performed to monitor single base additions of PNA initiated from (3-aminopropyl)triethoxysilane attached to the surface of PSi films, and mass spectrometry was conducted to verify synthesis of the desired sequence. Comparison of in situ synthesis to postsynthesis surface conjugation of the full PNA molecules showed that surface mediated, in situ PNA synthesis increased loading 8-fold. For therapeutic proof-of-concept, controlled PNA release from PSi films was characterized in phosphate buffered saline, and PSi nanoparticles fabricated from PSi films containing in situ grown PNA complementary to micro-RNA (miR) 122 generated significant anti-miR activity in a Huh7 psiCHECK-miR122 cell line. The applicability of this platform for biosensing was also demonstrated using optical measurements that indicated selective hybridization of complementary DNA target molecules to PNA synthesized in situ on PSi films. These collective data confirm that we have established a novel PNA–PSi platform with broad utility in drug delivery and biosensing.
登录
查看更多内容
影响因子：
56.9
作者：
Jopling, CL;Yi, MK;Sarnow, P
通讯作者：
Sarnow, P
影响因子：
--
作者：
Mehiri, Mohamed;Upert, Gregory;Patino, Nadia
通讯作者：
Patino, Nadia
影响因子：
4.9
作者：
Fang, Huafeng;Zhang, Ke;Taylor, John-Stephen A.
通讯作者：
Taylor, John-Stephen A.
影响因子：
19
作者：
Gaur, Girija;Koktysh, Dmitry S.;Weiss, Sharon M.
通讯作者：
Weiss, Sharon M.
DOI：
10.3390/s130404694
发表时间：
2013-04-09
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Pacholski C
通讯作者：
Pacholski C