Permeability characteristics of isolated perfused rat lungs.
Permeability characteristics of isolated perfused rat lungs.
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离体灌注大鼠肺的通透性特征。
DOI：
10.1152/jappl.1991.70.4.1854
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Chang,SW
中科院分区：
文献类型：
--
作者：
Czartolomna,J;Voelkel,NF;Chang,SW
我们研究了影响离体灌流大鼠肺的渗透性特征的因素，并比较了离体渗透性表面积产品（PS）与体内获得的。在用同种血液或含有4 g/100 ml白蛋白的生理盐溶液（PSS）灌注20 min的肺中，通过克恩等人[Am. J. Physiol.245（Heart Circ. Physiol.14）：H229-H236，1983]，分别为9.9 +/- 0.6（SE）和6.8 +/- 0.3 cm3.min-1.g湿肺-1.10（-2）。这些数值与在完整大鼠中获得的肺PS相似（7.7 +/- 0.4 cm3.min-1.g湿肺-1.10（-2）。在灌注肺，PS值的影响，灌注液白蛋白浓度，灌注时间的长度，血管募集的程度。肺隔离后20分钟，用含有Ficoll的无白蛋白PSS灌注的肺中的PS比用白蛋白-PSS灌注的肺中的PS高126%。此外，PS在Ficoll-PSS灌注肺增加甚至更高的灌注2小时后，PS的这种时间依赖性的增加被衰减的灌注液中加入0.1 g/100 ml白蛋白。用低氧（0或3%0（2））或高氧（95%0（2））气体混合物离体通气2小时不影响灌注肺的PS值。然而，PS升高离体肺灌注鱼精蛋白，这会导致内皮细胞损伤，或在大鼠体内暴露于人重组肿瘤坏死因子的肺。（250字处删节）
We examined the factors that influence the permeability characteristics of isolated perfused rat lungs and compared the ex vivo permeability-surface area product (PS) with that obtained in vivo. In lungs perfused for 20 min with homologous blood or a physiological salt solution (PSS) containing 4 g/100 ml albumin, mean PS values, obtained by the single-sample method of Kern et al. [Am. J. Physiol. 245 (Heart Circ. Physiol. 14): H229-H236, 1983], were 9.9 +/- 0.6 (SE) and 6.8 +/- 0.3 cm3.min-1.g wet lung-1.10(-2), respectively. These values were similar to lung PS obtained in intact rats (7.7 +/- 0.4 cm3.min-1.g wet lung-1.10(-2). In perfused lungs, PS values were influenced by the perfusate albumin concentration, the length of perfusion time, and the degree of vascular recruitment. Twenty minutes after lung isolation, PS was 126% higher in lungs perfused with albumin-free PSS containing Ficoll than in lungs perfused with albumin-PSS. Moreover, PS in Ficoll-PSS-perfused lungs increased even higher after 2 h of perfusion, and this time-dependent increase in PS was attenuated by addition of 0.1 g/100 ml albumin to the perfusate. Two hours of ex vivo ventilation with hypoxic (0 or 3% 0(2)) or hyperoxic (95% 0(2)) gas mixture did not affect PS values in perfused lungs. However, PS was elevated in lungs perfused ex vivo with protamine, which causes endothelial cell injury, or in lungs from rats exposed in vivo to human recombinant tumor necrosis factor.(ABSTRACT TRUNCATED AT 250 WORDS)