Granulocyte/macrophage colony-stimulating factor and interleukin-4-induced dendritic cells.
Granulocyte/macrophage colony-stimulating factor and interleukin-4-induced dendritic cells.
复制标题
粒细胞/巨噬细胞集落刺激因子和白介素 4 诱导的树突状细胞。
作者：
H. Hikino;K. Kasono;Masaki Kanzaki;Toshihiro Kai;F. Konishi;M. Kawakami
背景
我们研究了在诱导抗肿瘤免疫反应方面，GM-CSF/IL-4是否是分化树突状细胞(DC)的最有效的细胞因子组合。
材料和方法
建立了两种实验模型：携带MC38细胞的C57BL/6小鼠和携带恶病质诱导的结肠26细胞的Balb/c小鼠。肿瘤细胞裂解液冲击DC免疫后，皮下接种肿瘤。
结果
裂解液致敏的DC免疫C57BL/6小鼠后，可有效抵抗MC38的攻击，并可检测到MC38特异性的细胞毒性淋巴细胞(CTL)。然而，在Balb/c小鼠中，即使用裂解物冲击的DC免疫的组也没有表现出对Colon-26攻击的保护性免疫。出乎意料的是，与对照组相比，接种裂解物非脉冲DC的小鼠表现出恶病质进展的加速(p=0.031)。
结论
我们推测GM-CSF/IL-4诱导的DC促进了Balb/c小鼠以Th2为主的免疫。在肿瘤免疫治疗中，可以考虑哪种细胞因子的组合适合于DC的体外分化。
BACKGROUND
We investigated whether GM-CSF/IL-4 is the most efficient cytokine combination for differentiating dendritic cells (DC) in terms of its ability to elicit an antitumor immune response.
MATERIALS AND METHODS
Two experimental models were examined: C57BL/6 mice bearing MC38 cells and Balb/c mice bearing cachexia-inducible Colon-26 cells. After immunization with DC pulsed with whole tumor cell lysate, tumors were inoculated into the subcutis.
RESULTS
C57BL/6 mice immunized with lysate-pulsed DC effectively rejected the MC38 challenge and detectable MC38-specific cytotoxic lymphocytes (CTL) were observed. However, even those groups immunized with lysate-pulsed DC exhibited no protective immunity against Colon-26 challenge in Balb/c mice. Unexpectedly, mice inoculated with lysate-unpulsed DC showed an acceleration of cachectic progression (p=0.031) compared to control mice.
CONCLUSION
We speculate that GM-CSF/IL-4-induced DC promotes Th2-dominated immunity in Balb/c mice. Consideration might be given to which combination of cytokines is appropriate for the ex vivo differentiation of DC in tumor immunotherapy.
DOI：
10.1073/pnas.95.16.9482
发表时间：
1998-08-04
影响因子：
11.1
作者：
Fields, RC;Shimizu, K;Mulé, JJ
通讯作者：
Mulé, JJ