Phenotypic and functional characterization of T cells from patients with myasthenia gravis.
Phenotypic and functional characterization of T cells from patients with myasthenia gravis.
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重症肌无力患者 T 细胞的表型和功能特征。
DOI：
10.1172/jci114948
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Grob,D
中科院分区：
文献类型：
--
作者：
Mokhtarian,F;Pino,M;Ofosu-Appiah,W;Grob,D
对重症肌无力(MG)患者PBL细胞表面表型的研究表明，其T细胞中4B4+T细胞(辅助性/诱导性T细胞亚群)的比例明显高于年龄和性别匹配的对照组。MG患者PBL对纯化的乙酰胆碱受体(AChR)α链的反应明显高于正常对照组(NS)。88%的MG患者血清中存在抗AChR抗体，而NS患者血清中未检测到AChR抗体。用商陆丝裂原(69%)或AChR-α链(38%)刺激时，仅MG的PBL-B细胞能分泌抗AChR-α链的抗体，而单独的T、B细胞则不分泌抗体。在有重组IL-2存在和无AChR-α链存在的情况下，MG患者PBL中的T细胞比NS患者的T细胞更容易克隆。约50%的MG患者的T细胞克隆增殖到AChR-α链上，而NS患者则为零。这种反应是受人类白细胞抗原DR限制性的。与对照T细胞克隆相比，MG T细胞克隆没有表现出明显的细胞毒活性。我们的结果表明，在MG中，4B4+调节性T细胞在MG的发病机制中发挥作用，不是通过细胞毒性，而是通过它们刺激B细胞产生特异性抗体的能力。
A study of cell surface phenotypes of PBL of myasthenia gravis (MG) patients showed that their T cells had a significantly higher percentage of 4B4+ T cells (the helper/inducer subset) than age- and sex-matched controls. The PBL of MG patients proliferated significantly higher than those of normal subjects (NS) in response to the purified alpha chain of the acetylcholine receptor (AChR). Anti-AChR antibody was present in sera of 88% of MG and none of the NS. The PBL B cells from MG only, when cultured with autologous T cells and stimulated with either pokeweed mitogen (69%), or AChR-alpha chain (38%), secreted antibody to AChR-alpha chain, whereas T and B cells alone secreted no antibody. T cells from PBL of MG patients were more readily cloned than T cells of NS, by limiting dilution, in the presence of recombinant IL-2 and in the absence of AChR-alpha chain. About 50% of T cell clones from MG patients, compared to none from NS, proliferated to AChR-alpha chain. This response was HLA-DR restricted. MG T cell clones did not display significant cytotoxic activity, as compared to control T cell clones. Our results indicate that in MG, 4B4+ regulatory T cells play their role in the pathogenesis of MG, not by cytotoxicity, but more likely by their ability to stimulate specific antibody production by B cells.Images