Ectopic expression of Arabidopsis Target of Rapamycin (AtTOR) improves water-use efficiency and yield potential in rice.
Ectopic expression of Arabidopsis Target of Rapamycin (AtTOR) improves water-use efficiency and yield potential in rice.
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DOI：
10.1038/srep42835
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发表时间：
2017-02-23
影响因子：
4.6
通讯作者：
Kirti PB
中科院分区：
文献类型：
--
作者：
Bakshi A;Moin M;Kumar MU;Reddy AB;Ren M;Datla R;Siddiq EA;Kirti PB
雷帕霉素（TOR）是一种存在于所有真核生物中的多功能蛋白质，调节生长、发育、蛋白质翻译、核糖体生物合成、营养和能量信号传导。在本研究中，拟南芥TOR基因在广泛种植的籼稻中的异位表达导致在水分限制条件下增强植物生长，赋予农艺学上重要的水分利用效率（WUE）性状。AtTOR高表达水稻表现出丰富的分蘖，增加穗长，增加株高，高光合效率，叶绿素含量和低的叶绿素13 C。Δ 13 C在AtTOR高表达株系中仅为17 ‰，与高WUE呈负相关。这些品系对ABA介导的种子萌发抑制也不敏感。15个胁迫特异基因在高表达系中的显著上调表明它们对非生物胁迫耐受性的贡献。AtTOR的组成型表达也与推定的TOR复合物-1组分OsRaptor和OsLST 8的显著转录上调相关。葡萄糖介导的AtTOR基因转录激活促进侧根形成。综上所述，我们的研究结果表明，TOR，除了其多种细胞功能，也发挥了重要作用，在响应非生物胁迫和潜在的提高WUE和产量相关的属性。
The target of Rapamycin (TOR) present in all eukaryotes is a multifunctional protein, regulating growth, development, protein translation, ribosome biogenesis, nutrient, and energy signaling. In the present study, ectopic expression of TOR gene of Arabidopsis thaliana in a widely cultivated indica rice resulted in enhanced plant growth under water-limiting conditions conferring agronomically important water-use efficiency (WUE) trait. The AtTOR high expression lines of rice exhibited profuse tillering, increased panicle length, increased plant height, high photosynthetic efficiency, chlorophyll content and low ∆13C. Δ13C, which is inversely related to high WUE, was as low as 17‰ in two AtTOR high expression lines. These lines were also insensitive to the ABA-mediated inhibition of seed germination. The significant upregulation of 15 stress-specific genes in high expression lines indicates their contribution to abiotic stress tolerance. The constitutive expression of AtTOR is also associated with significant transcriptional upregulation of putative TOR complex-1 components, OsRaptor and OsLST8. Glucose-mediated transcriptional activation of AtTOR gene enhanced lateral root formation. Taken together, our findings indicate that TOR, in addition to its multiple cellular functions, also plays an important role in response to abiotic stress and potentially enhances WUE and yield related attributes.