The reduction in pigment epithelium-derived factor is a sign of malignancy in ovarian cancer expressing low-level of vascular endothelial growth factor
The reduction in pigment epithelium-derived factor is a sign of malignancy in ovarian cancer expressing low-level of vascular endothelial growth factor
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DOI：
10.1080/09513590802549841
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发表时间：
2009-01
影响因子：
2
通讯作者：
T. Tsuchiya;K. Nakahama;Y. Asakawa;T. Maemura;Masanobu Tanaka;S. Takeda;M. Morita;I. Morita
中科院分区：
文献类型：
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作者：
T. Tsuchiya;K. Nakahama;Y. Asakawa;T. Maemura;Masanobu Tanaka;S. Takeda;M. Morita;I. Morita
背景资料。血管生成是实体瘤进展和转移的关键因素。这项研究的目的是描述血管生成因子和抗血管生成因子在卵巢癌中的作用。方法：研究方法。采用实时定量聚合酶链式反应和Western blotting检测卵巢肿瘤组织中血管内皮生长因子(VEGF)和色素上皮生长因子(PEDF)的表达水平。肿瘤组织制备物高倍视野微血管总长度评价微血管密度(MVD)。结果。MVD与肿瘤的恶性程度相关。血管内皮生长因子(VEGF-H)表达最高的组织为恶性肿瘤。部分恶性肿瘤(血管内皮生长因子-L)的表达水平与良性肿瘤相当。因此，对PEDF的表达进行了检测。恶性血管内皮细胞生长因子在L恶性肿瘤中的表达水平明显低于良性肿瘤。另一方面，PEDF在恶性肿瘤组织中的表达水平与良性肿瘤组织中的表达水平无显著差异。结论。PEDF表达水平的降低可能是导致血管内皮生长因子-L卵巢肿瘤恶变的部分原因。此外，PEDF可能是判断卵巢肿瘤恶性程度的有用标记物。
Background. Angiogenesis is a critical factor in the progression of solid tumors and metastasis. The aim of this study was to characterise the roles of angiogenic and anti-angiogenic factors on ovarian cancer. Methods. The expression levels of vascular endothelial growth factor (VEGF, angiogenic factor) and pigment epithelial growth factor (PEDF, anti-angiogenic factor) were measured by real-time polymerase chain reaction and Western blotting in ovarian tumors. Microvessel density (MVD) was evaluated by the total microvessel length in high-power field of tumor tissue preparations. Results. MVD correlated with tumor malignancy. The tissues with the highest expression levels of VEGF (VEGF-H) were malignant tumors. The VEGF expression levels in some malignant tumors (VEGF-L) were as low as that in benign tumors. Therefore, the expression of PEDF was examined. The PEDF expression levels in VEGF-L malignant tumors were significantly lower than those in benign tumors. On the other hand, the PEDF expression levels in VEGF-H malignant tumor tissues were not significantly different from those in benign tumors. Conclusion. The reduction in PEDF expression levels may be, in part, responsible for tumor malignancy in VEGF-L ovarian tumors. Furthermore, PEDF may be a useful marker of malignancy in VEGF-L ovarian tumors.