Syndecan captures, protects, and transmits HIV to T lymphocytes
Syndecan captures, protects, and transmits HIV to T lymphocytes
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DOI：
10.1016/s1074-7613(02)00504-6
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发表时间：
2003-01-01
期刊：
影响因子：
32.4
通讯作者：
Gallay, PA
中科院分区：
文献类型：
--
作者：
Bobardt, MD;Saphire, ACS;Gallay, PA
这项研究表明，syndecan作为一种反式HIV受体发挥作用。我们发现，syndecan，当在非允许细胞中表达时，成为HIV吸附的主要介质。这种吸附是由gp120与多配体聚糖的硫酸乙酰肝素链结合介导的。虽然syndecan不能替代HIV进入受体，但它增强了广泛的灵长类慢病毒的反式感染性，包括从PBMC产生的初级病毒。此外，多配体蛋白聚糖可以保持病毒感染性一周，而未结合的病毒在不到一天的时间内就会失去感染性。此外，我们获得的证据表明，巨大的syndecan丰富的血管内皮衬里可以提供一个微环境，促进T细胞中的HIV复制。
This study demonstrates that syndecan functions as an in trans HIV receptor. We show that syndecan, when expressed in nonpermissive cells, becomes the major mediator for HIV adsorption. This adsorption is mediated by the binding of gp120 to the heparan sulfate chains of syndecan. Although syndecan does not substitute for HIV entry receptors, it enhances the in trans infectivity of a broad range of primate lentiviruses including primary viruses produced from PBMCs. Furthermore, syndecan preserves virus infectivity for a week, whereas unbound virus loses its infectivity in less than a day. Moreover, we obtain evidence suggesting that the vast syndecan-rich endothelial lining of the vasculature can provide a microenvironment which boosts HIV replication in T cells.