CYTOCHEMICAL-LOCALIZATION OF LYSOSOMAL ENZYMES IN RAT MEGAKARYOCYTES AND PLATELETS
CYTOCHEMICAL-LOCALIZATION OF LYSOSOMAL ENZYMES IN RAT MEGAKARYOCYTES AND PLATELETS
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DOI：
10.1172/jci108246
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发表时间：
1975-01-01
影响因子：
15.9
通讯作者：
BAINTON, DF
中科院分区：
文献类型：
--
作者：
BENTFELD, ME;BAINTON, DF
在血栓形成早期的“血小板释放反应”中，血小板会分泌溶酶。本研究旨在鉴定血小板的初级溶酶体，并确定其起源于巨核细胞。用电子显微镜和细胞化学方法，对正常大鼠和血小板减少症大鼠的巨核细胞和血小板中的两种溶酶体酶--芳基硫酸酯酶和酸性磷酸酶进行了定位。在血小板和成熟的巨核细胞中，这两种酶的反应产物仅限于175-250 nm的囊泡。这些小泡是初级溶酶，最初出现在可识别的最早的巨核细胞中，并在细胞成熟过程中数量增加。在未成熟和成熟的巨核细胞中，芳基硫酸酯酶和酸性磷酸酶也可以在类似高尔基体(GERL)的细胞器中表达，即单个光滑表面的脑池，在堆叠的高尔基体池附近有相关的小泡。高尔基体池和内质网中也可见少量酸性磷酸酶反应产物。在巨核细胞成熟的各个阶段的α颗粒中均未见反应产物，在血小板的α颗粒和5-羟色胺颗粒中也未见反应产物。因此，我们的发现表明，巨核细胞和血小板的原始溶酶体是巨核细胞分化早期来自GERL的小泡。它们只能在细胞化学染色后才能被识别，并且与α-和5-羟色胺颗粒不同。
Platelets secrete lysosmal enzymes during the "platelet release reaction" early in clot formation. This study was undertaken to identify primary lysosomes of platelets and to detemine their origin in megakaryocytes. Using electron microscopy and cytochemistry, we localized two lysosomal enzymes, arylsulfatase and acid phosphatase, in megakaryocytes and platelets of normal and thrombocytopenic rats. In platelets and mature megakaryocytes, reaction product for both enzymes is confined to vesicles measuring 175-250 nm. These vesicles, which are primary lysosmes, first appear in the earliest recognizable megakaryocytes and increase in number during cellular maturation. In immature and maturing megakaryocytes, arylsulfatase and acid phosphatase can also be demonstrated in an organell similar to GERL (Golgi-endoplasmic reticulumlysosome), i.e., single smooth-surfaced cisternal with associated vesicles near the stacked Golgi cisternae. Scant reaction product for acid phosphatase is also sometimes seen in Golgi cisternae and endoplasmic reticulum. No reaction product was found in alpha-granules at any stage of megakaryocyte maturation, nor in alpha- or serotonin granules of platelets. Thus, our findings indicate that the primay lysosomes of megakaryocytes and platelets are small vesicles derived from GERL early in megakaryocyte differentiation. They can be indentified only after cytochemical staining and are distinct from both alpha- and serotonin granules.Images