The structure of d(CGCGAAT[]TCGCG) . d(CGCGAATTCGCG); the incorporation of a thymine photodimer into a B-DNA helix.
The structure of d(CGCGAAT[]TCGCG) . d(CGCGAATTCGCG); the incorporation of a thymine photodimer into a B-DNA helix.
复制标题
d(CGCGAAT[]TCGCG) 的结构。
DOI：
10.1093/nar/12.11.4789
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发表时间：
1984
影响因子：
14.9
通讯作者：
Kollman,P
中科院分区：
文献类型：
--
作者：
Rao,SN;Keepers,JW;Kollman,P
鉴于胸腺嘧啶光二聚体的生物学意义，本文用分子力学方法研究了胸腺嘧啶二聚体在顺式构型中掺入和不掺入时十二核苷酸片段d（CGCGAATTCGCG）2的能量学。计算结果表明，胸腺嘧啶二聚体结构显示没有总的扭曲的双螺旋的构象变化相对于正常的B-DNA双螺旋主要限于二聚体区域。的dTp[ ]dT的能量揭示了一些构象，这是积极的几乎同样有利的，作为一个群体，定性与NMR研究这个分子。本文报道的构象研究结果的生物学复杂性已被visinedthecurrent可用的模型用于修复酶识别DNA“损伤”。
In the light of the biological significance of thymine photodimers, studies of the energetics of the dodecanucleotide fragment d(CGCGAATTCGCG)2have been carried out using the methods of molecular mechanics, with and without incorporation of a thymine dimer in thecis-synconfiguration. The results of the calculations suggest that the thymine dimerized structures show no gross distortion in the double helix with the conformational changes relative to the normal B-DNA double helix restricted largely to the dimer region. The energetics of dTp[ ]dT reveal a number of conformers which are energetically almost equally favorable and are, as a group, qualitatively consistent with NMR studies on this molecule. The biological omplications of the results of the conformational studies, reported here, have been examinedvis-a-visthe currently available models for the recognition of DNA “damage” by repair enzymes.