Comparative lipidomics analysis of HIV-1 particles and their producer cell membrane in different cell lines
Comparative lipidomics analysis of HIV-1 particles and their producer cell membrane in different cell lines
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DOI：
10.1111/cmi.12101
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发表时间：
2013-02-01
影响因子：
3.4
通讯作者：
Bruegger, Britta
中科院分区：
文献类型：
--
作者：
Lorizate, Maier;Sachsenheimer, Timo;Bruegger, Britta
人类免疫缺陷病毒1型（HIV-1）是一种逆转录病毒，它通过穿过受感染宿主细胞的质膜出芽获得其脂质包膜。各种研究表明，HIV-1膜与生产细胞质膜不同，表明病毒从预先存在的亚结构域出芽或病毒介导诱导专门的出芽膜。为了通过定量质谱法进行比较脂质组学分析，我们首先评估了分离细胞质膜的两种独立方法。随后的脂质分析质膜和HIV-1纯化的两种不同的细胞系显示了显着不同的脂质组成的病毒膜相比，宿主细胞质膜，独立的细胞类型的调查。病毒颗粒显着丰富的磷脂酰丝氨酸，鞘磷脂，己糖神经酰胺和饱和的磷脂酰胆碱物种相比，生产细胞的宿主细胞质膜，他们表现出降低水平的不饱和磷脂酰胆碱物种，磷脂酰乙醇胺和磷脂酰肌醇。观察到缩醛磷脂酰乙醇胺和磷脂酰甘油的HIV-1和供体质膜的脂质组成的细胞类型特异性差异，其仅在来自MT-4细胞的HIV-1中强烈富集。MT-4细胞来源的HIV-1也含有二氢鞘磷脂，如前所述，但这种脂质类也富集在宿主细胞膜上。综上所述，这些数据有力地支持了HIV-1选择特定的脂质环境进行形态发生的假设。
Human immunodeficiency virus type 1 (HIV-1) is a retrovirus that obtains its lipid envelope by budding through the plasma membrane of infected host cells. Various studies indicated that the HIV-1 membrane differs from the producer cell plasma membrane suggesting virus budding from preexisting subdomains or virus-mediated induction of a specialized budding membrane. To perform a comparative lipidomics analysis by quantitative mass spectrometry, we first evaluated two independent methods to isolate the cellular plasma membrane. Subsequent lipid analysis of plasma membranes and HIV-1 purified from two different cell lines revealed a significantly different lipid composition of the viral membrane compared with the host cell plasma membrane, independent of the cell type investigated. Virus particles were significantly enriched in phosphatidylserine, sphingomyelin, hexosylceramide and saturated phosphatidylcholine species when compared with the host cell plasma membrane of the producer cells; they showed reduced levels of unsaturated phosphatidylcholine species, phosphatidylethanolamine and phosphatidylinositol. Cell type-specific differences in the lipid composition of HIV-1 and donor plasma membranes were observed for plasmalogen-phosphatidylethanolamine and phosphatidylglycerol, which were strongly enriched only in HIV-1 derived from MT-4 cells. MT-4 cell-derived HIV-1 also contained dihydrosphingomyelin as reported previously, but this lipid class was also enriched in the host cell membrane. Taken together, these data strongly support the hypothesis that HIV-1 selects a specific lipid environment for its morphogenesis.