Toxicity and Biokinetics of Colloidal Gold Nanoparticles.
Toxicity and Biokinetics of Colloidal Gold Nanoparticles.
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DOI：
10.3390/nano5020835
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发表时间：
2015-05-21
期刊：
影响因子：
--
通讯作者：
Choi SJ
中科院分区：
文献类型：
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作者：
Jo MR;Bae SH;Go MR;Kim HJ;Hwang YG;Choi SJ
金纳米粒子具有广泛的生物学应用前景，但金纳米粒子是否具有潜在的体内外毒性尚未完全确定。在本研究中，在人肠细胞中以及在大鼠中14天重复口服给药后，评价了Au-NP的毒性。还进行了生物动力学研究，以评估口服吸收和组织分布。结果表明，金纳米粒子暴露24小时后，在细胞增殖抑制，膜损伤，氧化应激方面没有引起细胞毒性作用。然而，当少量细胞暴露于Au-NP 7天时，Au-NP处理的集落形成能力显着下降，表明其在高浓度长期暴露后的潜在毒性。生物动力学研究表明，金纳米粒子缓慢进入血液和轻微积累后，大鼠口服给药后，只有在肾脏。然而，口服给药的Au离子被迅速吸收，然后以高水平分布在肾、肝、肺和脾中，这表明Au-NP的生物命运主要是以纳米颗粒形式，而不是以离子Au形式。14天重复口服毒性评价显示，基于组织病理学、血液学和血清生化分析，Au-NP未引起大鼠严重毒性。
Gold nanoparticles (Au-NPs) have promising potential for diverse biological application, but it has not been completely determined whether Au-NP has potential toxicity in vitro and in vivo. In the present study, toxicity of Au-NP was evaluated in human intestinal cells as well as in rats after 14-day repeated oral administration. Biokinetic study was also performed to assess oral absorption and tissue distribution. The results demonstrated that Au-NP did not cause cytotoxic effects on cells after 24 h exposure in terms of inhibition of cell proliferation, membrane damage, and oxidative stress. However, when a small number of cells were exposed to Au-NP for seven days, colony forming ability remarkably decreased by Au-NP treatment, suggesting its potential toxicity after long-term exposure at high concentration. Biokinetic study revealed that Au-NP slowly entered the blood stream and slightly accumulated only in kidney after oral administration to rats. Whereas, orally administered Au ions were rapidly absorbed, and then distributed in kidney, liver, lung, and spleen at high levels, suggesting that the biological fate of Au-NP is primarily in nanoparticulate form, not in ionic Au. Fourteen-day repeated oral toxicity evaluation showed that Au-NP did not cause severe toxicity in rats based on histopathological, hematological, and serum biochemical analysis.