Genetic analysis of chikungunya viruses imported to mainland China in 2008.
Genetic analysis of chikungunya viruses imported to mainland China in 2008.
复制标题
DOI：
10.1186/1743-422x-7-8
复制
发表时间：
2010-01-18
期刊：
影响因子：
4.8
通讯作者：
Li D
中科院分区：
文献类型：
--
作者：
Zheng K;Li J;Zhang Q;Liang M;Li C;Lin M;Huang J;Li H;Xiang D;Wang N;Hong Y;Huang L;Li X;Pan D;Song W;Dai J;Guo B;Li D
基孔肯雅病毒（CHIKV）近年来在世界范围内引起了大规模暴发，特别是在印度洋和印度的岛屿上。该病毒由蚊子（埃及伊蚊）传播，这种蚊子在中国广泛存在，在中国南方人口密度特别高。对全长病毒序列的分析表明，获得了一个单一的适应性突变，为该物种传播CHIKV提供了选择优势。中国未报告因本地传播而暴发的病例，2008年以前中国大陆未发现输入病例。我们跟踪了中国南方地区输入性CHIKV的传播情况，分析了检测到的病毒的遗传特征，以评估其进化潜力。中国大陆首次发现猪流感病毒输入是在2008年。鉴定了4种输入病毒的基因组序列，系统发育分析表明，这些序列聚集在印度洋病毒群中；然而，在非结构蛋白编码区检测到7个氨基酸变化，在结构蛋白编码区检测到5个氨基酸变化。特别是在E2中检测到一个新的取代（K252Q），这可能影响CHIKV的神经毒力。在2例输入性基孔肯雅病病例中观察到E1的适应性突变A226V。报告了2008年到访中国的旅客中实验室确诊的CHIKV感染病例，病毒核酸和蛋白质的新突变可能代表了人类或蚊子宿主的适应性突变。
Chikungunya virus (CHIKV) has caused large outbreaks worldwide in recent years, especially on the islands of the Indian Ocean and India. The virus is transmitted by mosquitoes (Aedes aegypti), which are widespread in China, with an especially high population density in southern China. Analyses of full-length viral sequences revealed the acquisition of a single adaptive mutation providing a selective advantage for the transmission of CHIKV by this species. No outbreaks due to the local transmission of CHIKV have been reported in China, and no cases of importation were detected on mainland China before 2008. We followed the spread of imported CHIKV in southern China and analyzed the genetic character of the detected viruses to evaluate their potential for evolution. The importation of CHIKV to mainland China was first detected in 2008. The genomic sequences of four of the imported viruses were identified, and phylogenetic analysis demonstrated that the sequences were clustered in the Indian Ocean group; however, seven amino acid changes were detected in the nonstructural protein-coding region, and five amino acid changes were noted in the structural protein-coding regions. In particular, a novel substitution in E2 was detected (K252Q), which may impact the neurovirulence of CHIKV. The adaptive mutation A226V in E1 was observed in two imported cases of chikungunya disease. Laboratory-confirmed CHIKV infections among travelers visiting China in 2008 were presented, new mutations in the viral nucleic acids and proteins may represent adaptive mutations for human or mosquito hosts.