Genetic tagging shows increased frequency and longevity of antigen-presenting, skin-derived dendritic cells in vivo
Genetic tagging shows increased frequency and longevity of antigen-presenting, skin-derived dendritic cells in vivo
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DOI：
10.1038/ni962
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发表时间：
2003-09-01
影响因子：
30.5
通讯作者：
Jacob, J
中科院分区：
文献类型：
--
作者：
Garg, S;Oran, A;Jacob, J
树突状细胞(DC)是免疫反应的关键调节者，激活幼稚抗原特异性T淋巴细胞。据报道，在引流的淋巴结中，携带抗原的树突状细胞是罕见和短暂的。如此少量的短命DC如何能激活稀有的抗原特异性T细胞尚不清楚。在这里，我们显示，在用基因枪免疫小鼠皮肤后，携带抗原的DC迁移到引流淋巴结的数量比先前估计的高出100倍，并持续了大约2周。原位树突状细胞的频率和寿命确保了引流淋巴结中稀有的抗原特异性T细胞具有充分的抗原呈递和刺激作用。
Dendritic cells (DCs) are key regulators of immune responses that activate naive antigen-specific T lymphocytes. In draining lymph nodes, antigen-bearing DCs are reported to be rare and short-lived. How such small numbers of short-lived DCs can activate rare antigen-specific T cells is unclear. Here we show that after immunization of mouse skins by gene gun, the number of antigen-bearing DCs that migrate to draining lymph node is 100-fold higher than previously estimated and that they persist for approximately 2 weeks. The substantial frequency and longevity of DCs in situ ensures ample antigen presentation and stimulation for the rare antigen-specific T cells in draining lymph nodes.