Chronic wasting disease in a Wisconsin white-tailed deer farm
Chronic wasting disease in a Wisconsin white-tailed deer farm
复制标题
DOI：
10.1177/104063870802000534
复制
发表时间：
2008-09-01
影响因子：
1.5
通讯作者：
Samuel, Michael D.
中科院分区：
文献类型：
--
作者：
Keane, Delwyn P.;Barr, Daniel J.;Samuel, Michael D.
2002年9月，在威斯康星州的一个圈养农场的白尾鹿（Odocoileus virginianus）中，诊断出慢性消耗病（CWD），这是一种圈养和野生鹿科动物的朊病毒疾病。该设施随后被隔离，2006年1月，剩余的76只鹿被消灭。60只动物（79%）被发现是阳性的异常朊蛋白（朊蛋白（CWD））在至少一个组织的免疫组化染色;阳性染色的患病率很高，即使在年轻的鹿。虽然没有一只鹿在种群减少时表现出提示慢性消耗病的临床体征，但49只鹿在奥贝水平的髓质中有相当大的异常朊病毒积累。在59只鹿中观察到异常蛋白质的神经外蓄积，其中59只鹿中的58只（98%）在咽后淋巴结中蓄积，59只鹿中的56只（95%）在扁桃体中蓄积，58只鹿中的48只（83%）在直肠粘膜淋巴组织中蓄积。4只鹿的视网膜呈阳性，均在奥贝体中有明显的朊病毒积聚。一只鹿被认为是大脑中的PrP（CWD）阳性，但在神经组织中没有，这是白尾鹿的一个新观察结果。圈养鹿的感染率是野生鹿的20倍。虽然弱相关的神经组织中的感染率，朊病毒基因型是密切相关的进展朊病毒积累的obex。通过直肠肛门粘膜相关淋巴组织（或其他外周淋巴组织）活检进行的死前检测可能是扁桃体活检的有用辅助手段，用于监测有慢性消耗病感染风险的圈养畜群。
In September 2002, chronic wasting disease (CWD), a prion disorder of captive and wild cervids, was diagnosed in a white-tailed deer (Odocoileus virginianus) from a captive farm in Wisconsin. The facility was subsequently quarantined, and in January 2006 the remaining 76 deer were depopulated. Sixty animals (79%) were found to be positive by immunohistochemical staining for the abnormal prion protein (PrP(CWD)) in at least one tissue; the prevalence of positive staining was high even in young deer. Although none of the deer displayed clinical signs suggestive of CWD at depopulation, 49 deer had considerable accumulation of the abnormal prion in the medulla at the level of the obex. Extraneural accumulation of the abnormal protein was observed in 59 deer, with accumulation in the retropharyngeal lymph node in 58 of 59 (98%), in the tonsil in 56 of 59 (95%), and in the rectal mucosal lymphoid tissue in 48 of 58 (83%). The retina was positive in 4 deer, all with marked accumulation of prion in the obex. One deer was considered positive for PrP(CWD) in the brain but not in the extraneural tissue, a novel observation in white-tailed deer. The infection rate in captive deer was 20-fold higher than in wild deer. Although weakly related to infection rates in extraneural tissues, prion genotype was strongly linked to progression of prion accumulation in the obex. Antemortem testing by biopsy of rectoanal mucosal-associated lymphoid tissue (or other peripheral lymphoid tissue) may be a useful adjunct to tonsil biopsy for surveillance in captive herds at risk for CWD infection.