A pre-mRNA-binding protein accompanies the RNA from the gene through the nuclear pores and into polysomes
A pre-mRNA-binding protein accompanies the RNA from the gene through the nuclear pores and into polysomes
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DOI：
10.1016/s0092-8674(00)80980-0
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发表时间：
1996-01-26
期刊：
影响因子：
64.5
通讯作者：
Daneholt, B
中科院分区：
文献类型：
--
作者：
Visa, N;AlzhanovaEricsson, AT;Daneholt, B
在C.通过电子显微镜观察Balbiani环（BR）前体mRNA如何与蛋白质结合形成前体mRNP颗粒，这些颗粒如何移动到并穿过核孔，以及BR RNA如何参与细胞质中巨型多核糖体的形成。在这里，我们学习C。tentans hrp36，BR颗粒中丰富的蛋白质，并确定其与哺乳动物hnRNP A1相似。通过免疫电子显微镜观察，证明hrp 36在转录的同时被添加到BR RNA中，保留在核质BR颗粒中，并通过仍然与BR RNA结合的核孔转移。它出现在含有BR RNA的巨大多核糖体中，尽管正在进行翻译，但它仍然是一种丰富的蛋白质。
In the larval salivary glands of C. tentans, it is possible to visualize by electron microscopy how Balbiani ring (BR) pre-mRNA associates with proteins to form pre-mRNP particles, how these particles move to and through the nuclear pore, and how the BR RNA is engaged in the formation of giant polysomes in the cytoplasm. Here, we study C. tentans hrp36, an abundant protein in the BR particles, and establish that it is similar to the mammalian hnRNP A1. By immunoelectron microscopy it is demonstrated that hrp36 is added to BR RNA concomitant with transcription, remains in nucleoplasmic BR particles, and is translocated through the nuclear pore still associated with BR RNA. It appears in the giant BR RNA-containing polysomes, where it remains as an abundant protein in spite of ongoing translation.