Mapping of Ago2-GW182 functional interactions.
Mapping of Ago2-GW182 functional interactions.
复制标题
Ago2-GW182 功能相互作用的映射。
DOI：
10.1007/978-1-61779-046-1_4
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
Chan,EdwardKL
中科院分区：
文献类型：
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作者：
Yao,Bing;Li,Songqing;Lian,ShangLi;Fritzler,MarvinJ;Chan,EdwardKL
MicroRNA （miRNA）介导的基因表达转录后调控已成为了解许多生物过程中微调控制的主要焦点。Argonaute 2蛋白（Ago2）是rna诱导沉默复合体的核心成分，直接结合miRNA，在RNAi和miRNA两种通路上均发挥作用。GW182是GW小体（GWB，也称为哺乳动物p小体）的标记蛋白，已知与Ago2蛋白结合。这种Ago2 - gw182相互作用对于Ago2 - mirna介导的翻译沉默以及Ago2进入GWB至关重要。3'UTR报告基因对拴系Ago2的翻译沉默需要GW182才能发挥作用，而拴系GW182比拴系Ago2具有更强的抑制作用，显然不需要Ago2。本章详细描述了绘制Ago2-GW182相互作用的方法。
MicroRNA (miRNA)-mediated posttranscriptional regulation of gene expression has become a major focus in understanding fine-tuning controls in many biological processes. Argonaute 2 protein (Ago2), a core component of RNA-induced silencing complex, directly binds miRNA and functions in both RNAi and miRNA pathways. GW182 is a marker protein of GW bodies (GWB, also known as mammalian P-bodies) and is known to bind the Ago2 protein. This Ago2–GW182 interaction is crucial for Ago2–miRNA-mediated translational silencing as well as the recruitment of Ago2 into GWB. Translational silencing of tethered Ago2 to a 3′UTR reporter requires GW182 for function, whereas tethered GW182 exerts a stronger repression than tethered Ago2 and does not apparently require Ago2. This chapter describes in detail the methods used in mapping Ago2–GW182 interactions.