CDNA CLONE FOR THE HEAVY-CHAIN OF THE HUMAN B-CELL ALLOANTIGEN DC1 - STRONG SEQUENCE HOMOLOGY TO THE HLA-DR HEAVY-CHAIN
CDNA CLONE FOR THE HEAVY-CHAIN OF THE HUMAN B-CELL ALLOANTIGEN DC1 - STRONG SEQUENCE HOMOLOGY TO THE HLA-DR HEAVY-CHAIN
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DOI：
10.1073/pnas.79.20.6337
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
STROMINGER, JL
中科院分区：
文献类型：
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作者：
AUFFRAY, C;KORMAN, AJ;STROMINGER, JL
从富含HLA-DR序列的[人] B细胞mRNA组分构建c[互补]DNA文库，并通过差异筛选程序分离对应于在B淋巴细胞中特异表达的序列的cDNA克隆。用HLA-DR H链基因特异性探针对这些克隆进行分析，可以表征HLA-DR H链相关序列。一个克隆pDCH 1被证明编码DC 1 H链，因为从其核苷酸序列预测的氨基酸序列与DC 1 H链氨基末端序列中可用于比较的9个残基中的8个相匹配。DC 1同种异体抗原的H链由232个氨基酸组成，并可分为2个外部结构域，α 1（氨基酸1-87）和α 2（氨基酸88-181），连接肽（氨基酸182-194），疏水跨膜区（氨基酸195-217）和胞质内区（氨基酸218-232）。与HLA-DR H链的比较揭示了在第二外部Ig样结构域（α 2）和跨膜区中的强序列同源性。相比之下，第一个外部结构域、连接肽和胞质内区域保守性较低。
A c[complementary]DNA library was constructed from a [human] B cell mRNA fraction enriched for HLA-DR sequences, and cDNA clones corresponding to sequences specifically expressed in B lymphocytes were isolated by a differential screening procedure. Analysis of these clones with probes specific for the HLA-DR H chain gene allowed the characterization of HLA-DR H chain-related sequences. One clone, pDCH1, was demonstrated to encode the DC1 H chain because the amino acid sequence predicted from its nucleotide sequence matches 8 of 9 residues available for comparison in the amino-terminal sequence of the DC1 H chain. The H chain of the DC1 alloantigen is composed of 232 amino acids and can be divided into 2 external domains, .alpha.1 (amino acids 1-87) and .alpha.2 (amino acids 88-181), a connecting peptide (amino acids 182-194), a hydrophobic transmembrane region (amino acids 195-217), and an intracytoplasmic region (amino acids 218-232). Comparison with the HLA-DR H chain reveals strong sequence homology in the 2nd external Ig-like domain (.alpha.2) and the transmembrane region. In contrast, the 1st external domain, the connecting peptide, and the intracytoplasmic region are less conserved.