Four weeks of light-intensity exercise enhances empathic behavior in mice: The possible involvement of BDNF
Four weeks of light-intensity exercise enhances empathic behavior in mice: The possible involvement of BDNF
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DOI：
10.1016/j.brainres.2022.147920
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发表时间：
2022-04
期刊：
影响因子：
2.9
通讯作者：
Takeru Shima;Reika Kawabata-Iwakawa;Hayate Onishi;S. Jesmin;Tomonori Yoshikawa
中科院分区：
文献类型：
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作者：
Takeru Shima;Reika Kawabata-Iwakawa;Hayate Onishi;S. Jesmin;Tomonori Yoshikawa
移情是哺乳动物维持与他人关系的基本功能之一。身体活动有助于提高共情态度和行为，但运动强度对哺乳动物共情的影响尚不明确。在这里，我们测试了轻度运动对移情行为的影响，这种运动对大脑中神经营养因子的表达有有益的影响。8周龄雄性C57 BL/6小鼠进行强制轮跑，光照强度为7.0 m/min，30 min/d，5 d/周，持续4周。然后，所有的小鼠进行帮助行为，以评估他们的移情行为。收集岛叶皮质，分析mRNA和miRNA的表达。四周的低强度运动增强了助人行为。运动组小鼠的脑岛皮质BDNF基因表达高于久坐组小鼠。此外，岛叶皮质Fndc5和Bdnf的mRNA表达水平呈显著正相关。基于miRNA测序，51个miRNA中有26个在运动小鼠的岛叶皮层中显著上调，25个在运动小鼠的岛叶皮层中显著下调。51个miRNAs中有11个与助人行为显著相关，其中包含与FNDC5表达相关的miR-486a-3p。这些结果表明，岛叶皮层中的miR-486a-3p/Fndc5/Bdnf通路可能是治疗共情的靶点。
Empathy is one of the essential functions of mammals for maintaining relationships with others. Physical activity contributes to enhancing empathic attitude and behavior; however, it is remained to cover the effective intensity of exercise on mammal empathy. Here, we tested the effects of light-intensity exercise, which has beneficial effects on expressing neurotrophic factors in the brain, on empathic behavior. Eight-week-old male C57BL/6 mice were subjected to forced wheel running at light-intensity (7.0 m/min, 30 min/day, 5 days/week) for 4 weeks. Then, all mice were subjected to helping behavior to evaluate their empathic behavior. The insular cortex was collected for analyzing the expressions of mRNA and miRNA. Four weeks of light-intensity exercise enhanced helping behavior. Exercised mice exhibited higher Bdnf gene expressions in the insular cortex than sedentary mice. In addition, there was a significant positive correlation between mRNA levels of Fndc5 and Bdnf in the insular cortex. Based on miRNA sequencing, 26 out of 51 miRNAs were significantly upregulated, and 25 out of 51 miRNAs were significantly downregulated in the insular cortex of mice with exercise. There were significant correlations between 11 out of 51 miRNAs and helping behavior; miR-486a-3p, which relates to FNDC5 expression, was contained. These results imply that miR-486a-3p/Fndc5/Bdnf pathway in the insular cortex would be a possible target for treating empathy.