PHYSIOLOGY AND MORPHOLOGY OF MULTIRECEPTIVE NEURONS WITH C-AFFERENT FIBER INPUTS IN THE DEEP DORSAL HORN OF THE RAT LUMBAR SPINAL-CORD
PHYSIOLOGY AND MORPHOLOGY OF MULTIRECEPTIVE NEURONS WITH C-AFFERENT FIBER INPUTS IN THE DEEP DORSAL HORN OF THE RAT LUMBAR SPINAL-CORD
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DOI：
10.1152/jn.1987.58.3.460
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发表时间：
1987-09-01
影响因子：
2.5
通讯作者：
KING, AE
中科院分区：
文献类型：
--
作者：
WOOLF, CJ;KING, AE
细胞内记录技术已被用来研究神经元，响应于低和高强度的机械刺激的皮肤后爪（宽动态范围或多感受细胞）在深背角的第四腰椎段的脊髓，在去脑脊髓大鼠。电刺激坐骨神经中的A纤维在所有32个神经元中产生了短潜伏期反应。在32个神经元中，有28个神经元在坐骨神经无髓传入被激活时产生长潜伏期的延长兴奋。本文介绍了12个多感受性细胞的生理特性与A-和C-纤维的输入，其细胞体的位置建立辣根过氧化物酶离子电泳和形态的6个神经元在这一组中的细胞体位于椎板V。单一刺激坐骨神经的强度高到足以激活无髓传入纤维（C-纤维强度）产生两种模式的反应的神经元。在五个神经元的长潜伏期突触后电位（PSP）清楚地从短潜伏期A-纤维诱发的PSP分离产生，导致在早期放电，沉默期，和晚期放电。在7个神经元中发现的第二种模式是持续时间长的去极化，仅由C强度刺激产生，其从早期A纤维诱发的PSP持续，在100-200 ms达到峰值，并持续300-500 ms，在六种情况下产生连续的动作电位爆发，最大频率在C传入纤维输入的预期潜伏期，但没有明显的A-和C-动作电位的纤维诱发条带。这种突触后去极化足够大，可以抑制一个细胞的动作电位。重复刺激坐骨神经（1赫兹，10秒）在C-纤维强度产生五种不同类型的神经元的反应。在三个神经元的C-纤维PSP的大小和持续时间的逐步增加发生，导致动作电位（windup）的数量增加，而在两个，重复刺激导致在一个渐进的中度去极化的神经元和增加的动作电位诱发的总数在早期和晚期laveling。大的去极化，足以部分地抑制动作电位，在两个细胞的重复刺激过程中发展，有效地减少了每次刺激诱发的尖峰的数量。（400字处截断摘要）
Intracellular recording techniques have been used to study neurons that respond to low- and to high-intensity mechanical stimulation of the skin of the hindpaw (wide dynamic range or multireceptive cells) in the deep dorsal horn of the fourth lumbar segment of the spinal cord, in decerebrate-spinal rats. Electrical stimulation of the A-fibers in the sciatic nerve produced a short-latency response in all 32 neurons studied. A long-latency prolonged excitation was produced in 28 of the 32 neurons when the unmyelinated afferents in the sciatic nerve were activated. This paper describes the physiological properties of 12 multireceptive cells with A- and C-fiber inputs, whose cell body location was established by horseradish peroxidase ionophoresis and the morphology of six neurons in this group whose cell bodies lay within lamina V. Single stimuli to the sciatic nerve at an intensity high enough to activate unmyelinated afferent fibers (C-fiber strength) produced two patterns of response in the neurons. In five neurons a number of long-latency postsynaptic potentials (PSPs) clearly separated from the short-latency A-fiber evoked PSPs were produced, resulting in an early discharge, a silent period, and a late discharge. The second pattern, found in seven neurons, was a long-lasting depolarization, only generated by C-strength stimuli, which continued from the early A-fiber evoked PSPs, peaked at 100-200 ms, and lasted for 300-500 ms, producing in six cases a continuous burst of action potentials with a maximal frequency at the expected latency of the C-afferent fiber input but with no clear A- and C-fiber evoked banding of the action potentials. This postsynaptic depolarization was large enough to inactivate action potentials in one cell. Repeated stimuli to the sciatic nerve (1 Hz for 10 s) at C-fiber strength produced five different types of response in the neurons. In three neurons a progressive increase in the size and duration of the C-fiber PSPs occurred, resulting in an increase in the number of action potentials (windup), whereas in two, the repeated stimulation resulted in a progressive moderate depolarization of the neurons and an increase in the total number of action potentials evoked at both early and late latencies. Large depolarizations, sufficient to partially inactivate action potentials, developed during the repeated stimulation in two cells, effectively reducing the number of spikes evoked per stimulus.(ABSTRACT TRUNCATED AT 400 WORDS)