In vivo layer-resolved characterization of oral dysplasia via nonlinear optical micro-spectroscopy.
In vivo layer-resolved characterization of oral dysplasia via nonlinear optical micro-spectroscopy.
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DOI：
10.1364/boe.3.001579
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发表时间：
2012-07-01
影响因子：
3.4
通讯作者：
Vargas G
中科院分区：
文献类型：
--
作者：
Edward K;Qiu S;Resto V;McCammon S;Vargas G
光谱学已被证明是研究上皮组织肿瘤转化的一种强有力的技术。由于特定的层内癌前病变起源于口腔粘膜的分层，层分辨分析可能会提高我们对癌前转化机制的理解和临床诊断结果。然而，线性光谱中的天然荧光信号通常源自多层聚焦体积。在这项研究中，利用非线性光谱学在体内层分辨正常和发育异常组织之间的歧视的第一次。我们的研究结果揭示了许多层内的具体差异。
Optical spectroscopy has proven to be a powerful technique for studying neoplastic transformation in epithelial tissue. Since specific intra-layer precancerous changes originate in the stratified layers of the oral mucosa, layer-resolved analysis will likely improve both our understanding of the mechanism of premalignant transformation, and clinical diagnostic outcomes. However, the native fluorescence signal in linear spectroscopy typically originates from a multi-layered focal volume. In this study, nonlinear spectroscopy was exploited for in vivo layer-resolved discrimination between normal and dysplastic tissue for the first time. Our results revealed numerous intra-layer specific differences.